Team:Heidelberg/Project/Capsid Shuffling/ViroBytes
From 2010.igem.org
(→Protocols) |
(→ViroByte construction) |
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*perform ViroByte Phusion HiFi Hot Start PCR to obtain desired fragments with complementary ends | *perform ViroByte Phusion HiFi Hot Start PCR to obtain desired fragments with complementary ends | ||
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**98°C 30s 1x | **98°C 30s 1x | ||
**98°C 10s | **98°C 10s | ||
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- | *digestion with Bsa1 | + | *digestion with Bsa1 |
==='''Anchor preparation'''=== | ==='''Anchor preparation'''=== |
Revision as of 03:48, 28 October 2010
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