Team:Heidelberg/Notebook/miMeasure/August

From 2010.igem.org

July
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August
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October
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08/08/2010

seeding cells for test measurements - 96 well plate for plate-reader and FACS

test of media:

  • OptiMEM 0% FBS + Pen/Strep + L-Glu + other according to cell line
  • OptiMEM 2% FBS + Pen/Strep + L-Glu + other according to cell line
  • DMEM 10% FBS+ Pen/Strep + L-Glu + other according to cell line


  • cells did not adhere properly so we decided to coat 96-well plate with poly-L-lysine and repeat
  • significantly better growth in DMEM with 10% FBS, so we decided to grow cells in it and before measurement to change it to OptiMEM, to reduce high background fluorescence coming from FBS.