Team:Heidelberg/Modeling/miBSdesigner

(Difference between revisions)

Revision as of 19:13, 26 October 2010

BS designer for miRNA

Construction of microRNA binding sites

Sequence name

microRNA Sequence ( 5' -> 3')

Spacer (inert sequence)

Perfect Binding Site ?
Imperfect Binding Site with 4 nt bulge (9-12)
Personalized Binding Site

Seed sequence (1-8) ?

Customized MISMATCH position

Supplementary region ?

Customized MATCH positions

Modify AU content ?

A in position -1
A in position 1
A in position 9

A in position 0
A in position 8
A in position 10

Sticky ends for integration into plasmid ?

Customized:
5'
3'

Name of primer 1

Sequence of primer 1

Name of primer 2

Sequence of primer 2

@@ Line 49: / Line 49: @@
 				<td width=290px>
 					<div class="title">
-						Seed sequence (1-8) &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <span class="help"><a href="#">?</a></span>
+						Seed sequence (1-8) &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <span class="help"><a href="#" title="Seed sequence help">?</a></span>
 					</div>
 					<div class="option"><SELECT name="seed" onchange="chMd1()" disabled="disabled">