Team:Harvard/fences/parts

From 2010.igem.org

(Difference between revisions)
Line 47: Line 47:
<tr><td>Arp2mutForward</td><td>Point mutagenesis to remove restriction enzyme site</td><td>5'-CAA GAA GCT TGA CTT GTC TAC GAA CTC TTC CAT TTC-3'</td></tr>
<tr><td>Arp2mutForward</td><td>Point mutagenesis to remove restriction enzyme site</td><td>5'-CAA GAA GCT TGA CTT GTC TAC GAA CTC TTC CAT TTC-3'</td></tr>
<tr><td>Arp2mutReverse</td><td>Point mutagenesis to remove restriction enzyme site</td><td>5'-GAA ATG GAA GAA CTC GTA GAC AAG TCA AGC TCC TTG-3'</td></tr>
<tr><td>Arp2mutReverse</td><td>Point mutagenesis to remove restriction enzyme site</td><td>5'-GAA ATG GAA GAA CTC GTA GAC AAG TCA AGC TCC TTG-3'</td></tr>
 +
        <tr><td>Arp2mutForward</td><td>Point mutagenesis to remove restriction enzyme site</td><td>5'-CAA GAA GCT TGA CTT GTC TAC GAA CTC TTC CAT TTC-3'</td></tr>
 +
<tr><td>Bam.Gal4.Fwd</td><td>INSERT DESCRIPTION HERE</td><td>5'-AAA AGG ATC CAA AAA AAT GAA GCT ACT GTC TTC TAT C-3'</td></tr>
<tr><td>Bam.Gal4.Fwd</td><td>INSERT DESCRIPTION HERE</td><td>5'-AAA AGG ATC CAA AAA AAT GAA GCT ACT GTC TTC TAT C-3'</td></tr>
<tr><td>HmRXR.Hind.Rev</td><td>INSERT DESCRIPTION HERE</td><td>5'-AAT TAA GCT TGG ATC CCT CGA GCT GCA GC-3'</td></tr>
<tr><td>HmRXR.Hind.Rev</td><td>INSERT DESCRIPTION HERE</td><td>5'-AAT TAA GCT TGG ATC CCT CGA GCT GCA GC-3'</td></tr>

Revision as of 18:35, 3 August 2010


parts and primers

Vectors

NameOriginal NameDescriptionResistanceResistance (Plants)Sequence
V1 TEXT INSERT TEXT HERE Kanamycinpat
V2 TEXT INSERT TEXT HERE KanamycinnptII
V3 TEXT INSERT TEXT HERE Kanamycinpat
V4 TEXT INSERT TEXT HERE KanamycinnptII
V5 TEXT INSERT TEXT HERE KanamycinnptII


Primers

NameDescriptionSequence
Arp2mutForwardPoint mutagenesis to remove restriction enzyme site5'-CAA GAA GCT TGA CTT GTC TAC GAA CTC TTC CAT TTC-3'
Arp2mutReversePoint mutagenesis to remove restriction enzyme site5'-GAA ATG GAA GAA CTC GTA GAC AAG TCA AGC TCC TTG-3'
Arp2mutForwardPoint mutagenesis to remove restriction enzyme site5'-CAA GAA GCT TGA CTT GTC TAC GAA CTC TTC CAT TTC-3'
Bam.Gal4.FwdINSERT DESCRIPTION HERE5'-AAA AGG ATC CAA AAA AAT GAA GCT ACT GTC TTC TAT C-3'
HmRXR.Hind.RevINSERT DESCRIPTION HERE5'-AAT TAA GCT TGG ATC CCT CGA GCT GCA GC-3'
LCRXRINSERT DESCRIPTION HERE5'-GAT GAA GCT TGG ATC CCT CGA GCT GC-3'
VP16.Hind.FwdINSERT DESCRIPTION HERE5'-AAA AAA GCT TAA AAA AAT GGC TAG CGC CGC CAC CAT G-3'
VP16.RevINSERT DESCRIPTION HERE5'-CTG CAG CGG CCG CTA CTA GTG CCC TTG GAA TTG ACG AGT AC-3'
NLS.serine.BB.FwdINSERT DESCRIPTION HERE5'-/5Phos/CTA GAA GTT CTG TTG TTC ATC CTA AGA AGA AGA GAA AGG TTT GAA CTA GTA GCG GCC GCT GCA-3'
NLS.serine.BB.RevINSERT DESCRIPTION HERE5'-/5Phos/GCG GCC GCT ACT AGT TCA AAC CTT TCT CTT CTT CTT AGG ATG AAC AAC AGA ACT T-3'
Fwd.BB.AtEXP2for amplification/extraction of an arabidopsis germination-specific promoter5'-CCT TTC TAG ACC TGG TGT GGT TTC TTT G-3'
Rev.BB.AtEXP2for amplification/extraction of an arabidopsis germination-specific promoter5'-AAG GCT GCA GCG GCC GCT ACT GAT ATT TGC TGA TGG GCT AAA G-3'
Fwd.BB.AtARP2for amplification/extraction of the arabidopsis ARP2 promoter5'-CCT TTC TAG ACC ATC ACA TAT TTG TAG AAT G-3'
Rev.BB.AtARP2for amplification/extraction of the arabidopsis ARP2 promoter5'-AAG GCT GCA GCG GCC GCT ACT AGT CTT CTC CGA TTT CTA TAG AG-3'
barnase2.BB.fwdaddition of biobrick sites/ends to barnase gene5'-CCT TTC TAG AAT GGT ACC GGT TAT CAA CAC G-3'
BB.Barnase.Revaddition of biobrick sites/ends to barnase gene5'-AAG GCT GCA GCG GCC GCT ACT AGT TTA TCT GAT TTT TGT AAA GGT CTG-3'
NLS.BB.Fwdaddition of biobrick sites/ends to the arabidopsis NLS5'-/5Phos/CTA GAT CTT CTG TTG TTC ATC CAT AGA AGA AGA GAA AGG TTT GAA CTA GTA GCG GCC GCT GCA-3'
NLS.BB.Revaddition of biobrick sites/ends to the arabidopsis NLS5'/5Phos/GCG GCC GCT ACT AGT TCA AAC CTT TCT CTT CTT CTT AGG ATG AAC AAC AGA AGA-3'
LacIn.BB.FwdINSERT DESCRIPTION HERE5'-CCT TGA ATT CGC GGC CGC ATC TAG AAT GAA AC AGT AAC GTT ATA CGA TGT C-3'
LacIN.BB.RevINSERT DESCRIPTION HERE5' AAG GTC GCA GCA GCG GCC GCT ACT AGT TCA AAC CTT TCT CTT CTT CTT AGG ATG AAC AAC AGA AGA CTG CCC GCT TTC CAG TCG GGA AA-3'
BB.Gal3DBD.FwdINSERT DESCRIPTION HERE5'-CTT TTC TAG AAT GAA GCT ACT GTC TTC TAT C-3'
BB.Gal4DBD.RevINSERT DESCRIPTION HERE5'-AAG GCT GCA GCG GCC GCT ACT AGT CGA TAC AGT CAA CTG TCT TTG-3'
BB.Barstar.Fwdaddition of biobrick sites/ends to barstar gene5'CCT TTC TAG AAT GAA AAA AGC AGT CAT TAA C-3'
BB.Barstar.Revaddition of biobrick sites/ends to barstar gene5'-AAG GCT GCA GCG GCC GCT ACT AGT TTA AGA AAG TATA GAT GGT GAT GTC-3'
2 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP283 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP294 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP305 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP331 of 2 for sequencing V7 and V8 biobrick cloning site
VP342 of 2 for sequencing V7 and V8 biobrick cloning site