Team:Harvard/fences/parts

From 2010.igem.org

(Difference between revisions)
Line 46: Line 46:
<tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Sequence</b></td></tr>
<tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Sequence</b></td></tr>
<tr><td>Arp2mutForward</td><td>Point mutagenesis to remove restriction enzyme site</td><td>5'-CAA GAA GCT TGA CTT GTC TAC GAA CTC TTC CAT TTC-3'</td></tr>
<tr><td>Arp2mutForward</td><td>Point mutagenesis to remove restriction enzyme site</td><td>5'-CAA GAA GCT TGA CTT GTC TAC GAA CTC TTC CAT TTC-3'</td></tr>
-
<tr><td>VP4</td><td>pENTCUP2-biobrick multiple cloning site-NheI reverse primer for Expression Series insert</td><td></td></tr>
+
<tr><td>Arp2mutReverse</td><td>Point mutagenesis to remove restriction enzyme site</td><td>5'-GAA ATG GAA GAA CTC GTA GAC AAG TCA AGC TCC TTG-3'</td></tr>
<tr><td>VP5</td><td>HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert</td><td></td></tr>
<tr><td>VP5</td><td>HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert</td><td></td></tr>
<tr><td>VP6</td><td>gusA-NheI reverse primer for Reporter Series 1 insert</td><td></td></tr>
<tr><td>VP6</td><td>gusA-NheI reverse primer for Reporter Series 1 insert</td><td></td></tr>

Revision as of 17:56, 3 August 2010


parts and primers

Vectors

NameOriginal NameDescriptionResistanceResistance (Plants)Sequence
V1 TEXT INSERT TEXT HERE Kanamycinpat
V2 TEXT INSERT TEXT HERE KanamycinnptII
V3 TEXT INSERT TEXT HERE Kanamycinpat
V4 TEXT INSERT TEXT HERE KanamycinnptII
V5 TEXT INSERT TEXT HERE KanamycinnptII


Primers

NameDescriptionSequence
Arp2mutForwardPoint mutagenesis to remove restriction enzyme site5'-CAA GAA GCT TGA CTT GTC TAC GAA CTC TTC CAT TTC-3'
Arp2mutReversePoint mutagenesis to remove restriction enzyme site5'-GAA ATG GAA GAA CTC GTA GAC AAG TCA AGC TCC TTG-3'
VP5HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert
VP6gusA-NheI reverse primer for Reporter Series 1 insert
VP7HindIII-biobrick multiple cloning site-smGFP-forward primer for Reporter Series 1 insert
VP8smGFP-NheI reverse primer for Reporter Series 1 insert
VP9Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert
VP11Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert
VP121 of 5 for sequencing V12 biobrick cloning site and smgfp reporter
VP132 of 5 for sequencing V12 biobrick cloning site and smgfp reporter
VP143 of 5 for sequencing V12 biobrick cloning site and smgfp reporter
VP154 of 5 for sequencing V12 biobrick cloning site and smgfp reporter
VP165 of 5 for sequencing V12 biobrick cloning site and smgfp reporter
VP171 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP182 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP193 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP204 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP215 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP226 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP237 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP248 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP259 of 9 for sequencing V11 biobrick cloning site and gusA reporter
VP261 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP272 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP283 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP294 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP305 of 5 for sequencing V9 and V10 biobrick cloning site and promoter
VP331 of 2 for sequencing V7 and V8 biobrick cloning site
VP342 of 2 for sequencing V7 and V8 biobrick cloning site