Team:Harvard/color/parts

From 2010.igem.org

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<h2>RNAi constructs</h2>
<h2>RNAi constructs</h2>
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<table cellspacing="0">
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<tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Size</b></td><td><b>Sequence</b></td></tr>
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<tr><td><b>Biobrick Name</b></td><td><b>Description</b></td><td><b>Size</b></td><td><b>Sequence</b></td></tr>
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<tr><td>BBa_K382010</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2)knockdown</td><td>413</td><td></td></tr>
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<tr><td>BBa_K382010</td><td>amiRNA construct for lycopene epsilon cyclase (LUT2)knockdown</td><td>413</td><td><a href="https://2010.igem.org/Team:Harvard/c1">link</a></td></tr>
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<tr><td>BBa_K382011</td><td>amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown</td><td>413</td><td><a href="https://2010.igem.org/Team:Harvard/c2">link</a></td></tr>
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<tr><td>BBa_K382012</td><td>amiRNA construct for lycopene beta cyclase (LYC) knockdown</td><td>413</td><td><a href="https://2010.igem.org/Team:Harvard/c3">link</a></td></tr>
</table>
</table>
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<h2>Primers</h2>
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<tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Sequence</b></td></tr>
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<tr><td>CP1</td><td>LUT2 I</td><td>gaTAACTTCCTAGTAACCCGCATtctctcttttgtattcc</td></tr>
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<tr><td>CP2</td><td>LUT2 II</td><td>gaATGCGGGTTACTAGGAAGTTAtcaaagagaatcaatga</td></tr>
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<tr><td>CP3</td><td>LUT2 III</td><td>gaATACGGGTTACTACGAAGTTTtcacaggtcgtgatatg</td></tr>
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<tr><td>CP4</td><td>LUT2 IV</td><td>gaAAACTTCGTAGTAACCCGTATtctacatatatattcct</td></tr>
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<tr><td>CP13</td><td>BETA-OHASE I</td><td>gaTCGTTTAAATCGAAGAGACGCtctctcttttgtattcc</td></tr>
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<tr><td>CP14</td><td>BETA-OHASE II</td><td>gaGCGTCTCTTCGATTTAAACGAtcaaagagaatcaatga</td></tr>
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<tr><td>CP15</td><td>BETA-OHASE III</td><td>gaGCATCTCTTCGATATAAACGTtcacaggtcgtgatatg</td></tr>
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<tr><td>CP16</td><td>BETA-OHASE IV</td><td>gaACGTTTATATCGAAGAGATGCtctacatatatattcct</td></tr>
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<tr><td>CP9</td><td>LYC I</td><td>gaTAATTATCCAACGACTTGCAGtctctcttttgtattcc</td></tr>
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<tr><td>CP10</td><td>LYC II</td><td>gaCTGCAAGTCGTTGGATAATTAtcaaagagaatcaatga</td></tr>
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<tr><td>CP11</td><td>LYC III</td><td>gaCTACAAGTCGTTGCATAATTTtcacaggtcgtgatatg</td></tr>
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<tr><td>CP12</td><td>LYC IV</td><td>gaAAATTATGCAACGACTTGTAGtctacatatatattcct</td></tr>
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</table>
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Latest revision as of 00:25, 27 October 2010



parts and primers

RNAi constructs

Biobrick NameDescriptionSizeSequence
BBa_K382010amiRNA construct for lycopene epsilon cyclase (LUT2)knockdown413link
BBa_K382011amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown413link
BBa_K382012amiRNA construct for lycopene beta cyclase (LYC) knockdown413link


Primers

NameDescriptionSequence
CP1LUT2 IgaTAACTTCCTAGTAACCCGCATtctctcttttgtattcc
CP2LUT2 IIgaATGCGGGTTACTAGGAAGTTAtcaaagagaatcaatga
CP3LUT2 IIIgaATACGGGTTACTACGAAGTTTtcacaggtcgtgatatg
CP4LUT2 IVgaAAACTTCGTAGTAACCCGTATtctacatatatattcct
CP13BETA-OHASE IgaTCGTTTAAATCGAAGAGACGCtctctcttttgtattcc
CP14BETA-OHASE IIgaGCGTCTCTTCGATTTAAACGAtcaaagagaatcaatga
CP15BETA-OHASE IIIgaGCATCTCTTCGATATAAACGTtcacaggtcgtgatatg
CP16BETA-OHASE IVgaACGTTTATATCGAAGAGATGCtctacatatatattcct
CP9LYC IgaTAATTATCCAACGACTTGCAGtctctcttttgtattcc
CP10LYC IIgaCTGCAAGTCGTTGGATAATTAtcaaagagaatcaatga
CP11LYC IIIgaCTACAAGTCGTTGCATAATTTtcacaggtcgtgatatg
CP12LYC IVgaAAATTATGCAACGACTTGTAGtctacatatatattcct

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