Team:Groningen/Transformation to E. coli

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Transformation to E. coli

  • Add 10 µL of ligate to 100 µL competent cells
  • Put cells for 30 minutes on ice
  • Heat shock the cells for 45 seconds at 42 °C
  • Put cells on ice for 2 minutes
  • Add 400 µL LB and grow for 1 hour at 37 °C
  • Plate 100 µL of cells on LB agar (2%) plates