Team:Groningen/Home

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The International Genetically Engineered Machine Competition is a annually held synthetic biology competition for undergraduates from all over the world. Teams aim to combine biology and engineering so that they are able to design, create and operate biological circuits within living organisms. Important in this iGEM field of synthetic biology are of course the projects standing on themselves, but synthetic biology is about more than biological systems only: using modelling techniques hypothetical systems can be explored or existing systems optimized, human perspectives are important to asses possibilities of implementing systems in our society and education will help the broader public to gain an understanding of the how's, why's and what's of synthetic biology.
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<a href="http://2010.igem.org/Team:Groningen#/project"><img src="http://2010.igem.org/wiki/images/d/dd/Groningen-Reel-Stage1.jpg" alt="" title="#biofilm-caption" /></a>
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<a href="http://2010.igem.org/Team:Groningen#/project"><img src="http://2010.igem.org/wiki/images/d/d3/Groningen-Reel-Stage2.jpg" alt="" title="#chaplin-caption" /></a>
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<a href="http://2010.igem.org/Team:Groningen#/project"><img src="http://2010.igem.org/wiki/images/6/6f/Groningen-Reel-Stage3.jpg" alt="" title="#killswitch-caption" /></a>
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But most of all iGEM is about fun, creativity and thinking out of the box. That's why the team of Groningen is excited to get started on our project for this years iGEM competition. Our team of 12 undergraduate students will try to create some new perspectives on biological engineering by creating a self assembling hydrophobic biofilm using ''Bacillus subtilis'' colonies.
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For more info, check the [http://2010.igem.org/Main_Page iGEM website] and our first updates on [http://2010.igem.org/Team:Groningen#/project the project] or [http://2010.igem.org/Team:Groningen#/images pictures of our progress].
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<div id="biofilm-caption" class="nivo-html-caption">
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  A self assembling bio-based coating is form, a </html>[http://2010.igem.org/Team:Groningen#/biofilm rigid biofilm]<html>.
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<div id="chaplin-caption" class="nivo-html-caption">
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    </html>[http://2010.igem.org/Team:Groningen#/expression Expression]<html> of hydrophobic proteins called </html>[http://2010.igem.org/Team:Groningen#/hydrophobins chaplins]<html> is induced by the biofilm causing strong surface hydrophobicity.  
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    The strongly hydrophobic biofilm will die off by a </html>[http://2010.igem.org/Team:Groningen#/killswitch_model killswitch]<html>, leaving a nice hydrophobic biological coating.
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==Self assembling hydrophobic biofilm==
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We aim to design a biological coating as an alternative to for example chemical coatings. For this we, unconventionally, utilized a ''Bacillus subtilis'' [http://2010.igem.org/Team:Groningen#/biofilm biofilm]. We wanted to enable our biofilm to be equipped with an interesting property which is automatically initiated. So we introduced an [http://2010.igem.org/Team:Groningen#/expression expression trigger] which relies on quorum sensing. Our project was directed at finding an alternate solution to biofouling, since regular, chemical coatings which are widely in use pose a threat to the environment. In nature the lotus leaves show self-cleansing properties ascribed to their extreme surface hydrophobicity. In the prokaryotic domain we stumbled upon [http://2010.igem.org/Team:Groningen#/hydrophobins chaplins], strongly hydrophobic proteins originating from ''Streptomyces coelicolor''. Surface hydrophobicity is a very useful property and is used in many [http://2010.igem.org/Team:Groningen#/applications applications] ranging from not only antifouling coatings but also other applications which require water repellence to applications in the field of medical sciences. During our project we have contributed to the parts registry whit numerous [http://2010.igem.org/Team:Groningen#/biobricks BioBricks].
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We believe that there is a great future for biological coatings as demonstrated within the project. Hydrophobic biological coatings can provide a greener antifouling solution. However using the underlying mechanisms of biofilm triggered expression for other systems like dynamic painting, sensing of environmental changes or even the integration with silicon chips might be within the realm of possibilities. So we challenge coming iGEM teams to further explore and [http://2010.igem.org/Team:Groningen#/brainstorm brainstorm] about the possibilities of using biofilms as a host for a wide range of applications.
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<a href="http://2010.igem.org/Team:Groningen#/team"><img src="http://2010.igem.org/wiki/images/f/f5/Groningen-Home-Team.jpg" alt="Team"></a>
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This year a [http://2010.igem.org/Team:Groningen#/team team] of young inspired undergraduates from the [http://www.rug.nl University of Groningen] participated in the amazing challenge of iGEM. A multi-disciplinary team of Molecular Biologists, Chemists, Computer Scientists, Journalists and others spend the summer creating a wonderful project in the emerging field of synthetic biology
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<div><a href="http://2010.igem.org/Team:Groningen#/modeling"><img src="http://2010.igem.org/wiki/images/1/1e/Groningen-Home-Model.jpg" alt="Modeling"></a><div></html>
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Using computer models we worked on the frontiers of knowledge. [http://2010.igem.org/Team:Groningen#/expression_model Gene expression] was simulated and a simple explanation for [http://2010.igem.org/Team:Groningen#/biofilm_model cell differentiation] was proposed. Also aiding in ethics and practical feasibility a [http://2010.igem.org/Team:Groningen#/killswitch_model kill switch] system was studied. Finally a [http://2010.igem.org/Team:Groningen#/info_standard new standard] was proposed for characterizing Biobrick parts so future can be streamlined.
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<div><a href="http://2010.igem.org/Team:Groningen#/practices"><img src="http://2010.igem.org/wiki/images/7/77/Groningen-Home-Human.jpg" alt="Human Practices"></a><div></html>
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Because we believe that synthetic biology can better the lives of people and ensure long term prosperity for all humans we spend time [http://2010.igem.org/Team:Groningen#/practices educating] high school students. But not all is perfect so [http://2010.igem.org/Team:Groningen#/safety risks] were assessed and we philosophized on the [http://2010.igem.org/Team:Groningen#/ethics ethical] aspects of synthetic biology.
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=== Our sponsors ===
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{{Team:Groningen/sponsors}}
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Latest revision as of 01:32, 28 October 2010

A self assembling bio-based coating is form, a rigid biofilm.
Expression of hydrophobic proteins called chaplins is induced by the biofilm causing strong surface hydrophobicity.
The strongly hydrophobic biofilm will die off by a killswitch, leaving a nice hydrophobic biological coating.

Self assembling hydrophobic biofilm

We aim to design a biological coating as an alternative to for example chemical coatings. For this we, unconventionally, utilized a Bacillus subtilis biofilm. We wanted to enable our biofilm to be equipped with an interesting property which is automatically initiated. So we introduced an expression trigger which relies on quorum sensing. Our project was directed at finding an alternate solution to biofouling, since regular, chemical coatings which are widely in use pose a threat to the environment. In nature the lotus leaves show self-cleansing properties ascribed to their extreme surface hydrophobicity. In the prokaryotic domain we stumbled upon chaplins, strongly hydrophobic proteins originating from Streptomyces coelicolor. Surface hydrophobicity is a very useful property and is used in many applications ranging from not only antifouling coatings but also other applications which require water repellence to applications in the field of medical sciences. During our project we have contributed to the parts registry whit numerous BioBricks.


We believe that there is a great future for biological coatings as demonstrated within the project. Hydrophobic biological coatings can provide a greener antifouling solution. However using the underlying mechanisms of biofilm triggered expression for other systems like dynamic painting, sensing of environmental changes or even the integration with silicon chips might be within the realm of possibilities. So we challenge coming iGEM teams to further explore and brainstorm about the possibilities of using biofilms as a host for a wide range of applications.

Team
This year a team of young inspired undergraduates from the University of Groningen participated in the amazing challenge of iGEM. A multi-disciplinary team of Molecular Biologists, Chemists, Computer Scientists, Journalists and others spend the summer creating a wonderful project in the emerging field of synthetic biology
Modeling
Using computer models we worked on the frontiers of knowledge. Gene expression was simulated and a simple explanation for cell differentiation was proposed. Also aiding in ethics and practical feasibility a kill switch system was studied. Finally a new standard was proposed for characterizing Biobrick parts so future can be streamlined.
Human Practices
Because we believe that synthetic biology can better the lives of people and ensure long term prosperity for all humans we spend time educating high school students. But not all is perfect so risks were assessed and we philosophized on the ethical aspects of synthetic biology.


Our sponsors

Sigma Aldrich DSM NGI GBB Kluyver Center Bioké Stichting biotechnology Nederland (SBN) 101 promotions Center for Synthetic Biology