Team:Groningen/Biobricks

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Biobricks

Here is our registry of our biobricks: <groupparts>iGEM010 Groningen</groupparts>

Summary:

Below is given a short description of all the biobricks in our registry.

Chaplins

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K305001 BBa_K305001] Hydrophobic protein chaplin C (chpC)

Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K305002 BBa_K305002] chaplin C (chpDC) with modified sortase binding motive

Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.

Modified C-terminal Sortase(SrtA from S. aureus.) recognition site.

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K305003 BBa_K305003] Hydrophobic protein chaplin E (chpE)

Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K305004 BBa_K305004] Hydrophobic protein chaplin H (chpH)

Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K305005 BBa_K305005] Sortase srtA

Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall

Promotors

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K305006 BBa_K305006] yqxMpr

yqxM promotor effective in biofilm formation, regulates tasA production

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K305007 BBa_K305007] srfApr 1

Promotor for the surfactin regulatory gene. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K305008 BBa_K305008] srfApr 2

Promotor for the surfactin regulatory gene.

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K305009 BBa_K305009] srfApr1>GFP

Promotor for the surfactin regulatory gene with GFP.

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K305010 BBa_K305010] srfApr2>GFP

Promotor for the surfactin regulatory gene with GFP.

Plasmid Backbone

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K305011 BBa_K305011] Subtilin inducible expression plasmid for Bacillus subtilis

Adapted from the system for subtilin-regulated gene expression (SURE) in Bacillus subtilis [1], this plasmid was constructed to express proteins present in RCF 10 BioBricks upon subtilin induction.