Team:Groningen/12 July 2010

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'''Week 28, Arend Jan'''
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A mix of the ChpE and ChpH genes were ligated into the pNZ8901-bbs backbone and a restriction check was performed on 8 clones. ChpE contains a DpnI site whereas ChpH does not. A restriction with DpnI and XhoI should yield a 487bp and 176bp fragment for ChpE and just a 648bp fragment for ChpH (+ a ~3500bp backbone fragment for both)
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<pre>- 10ul plasmid
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- 1.5ul buffer R
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- 0.5ul DpnI
 +
- 0.5ul XhoI
 +
- 2.5ul MQ
 +
 +
 +
[[Image:13-07-10gn.jpg|200px|thumb|left|]]
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<br style="clear: both" />
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These ligations are performed with the backbone that still contains unwanted IS element with the PstI site. At the same time we are attempting to delete this element using a related backbone.

Revision as of 16:12, 23 October 2010

iGEM Groningen 2010

Hydrophobofilm
pushing coatings into a greener future

Week 28, Arend Jan


A mix of the ChpE and ChpH genes were ligated into the pNZ8901-bbs backbone and a restriction check was performed on 8 clones. ChpE contains a DpnI site whereas ChpH does not. A restriction with DpnI and XhoI should yield a 487bp and 176bp fragment for ChpE and just a 648bp fragment for ChpH (+ a ~3500bp backbone fragment for both)

- 10ul plasmid 
- 1.5ul buffer R
- 0.5ul DpnI
- 0.5ul XhoI
- 2.5ul MQ


[[Image:13-07-10gn.jpg|200px|thumb|left|]]
<br style="clear: both" />


These ligations are performed with the backbone that still contains unwanted IS element with the PstI site. At the same time we are attempting to delete this element using a related backbone.





{{Team:Groningen/Footer}}