Team:GeorgiaTech/Systems Modeling

From 2010.igem.org

(Difference between revisions)
Line 78: Line 78:
</table>
</table>
-
<center><img src="https://static.igem.org/mediawiki/2010/a/a4/Modelingpic_1.png" alt="modeling picture"</center>
+
<p><center><font color="#FFFFFF" size=5><b>Modeling Bacterial Heat Production Due to AOX Pathway/b></font></center></p>
-
<p>
+
<center><table width="900" border="0" cellpadding="2" cellspacing="10">
-
<p>
+
  <tr>
-
Figure (A) depicts the approach taken to model E. coli heat production and localization using AOX in a 'dry' plated culture. In order to determine the steady state temperature of E. coli colonies expressing heat, colonies are approximated as a single layer composed of a certain amount of heat producing cells with thermal conductivity k1. The heat produced in this situation is transferred by conduction with the media (agar) below the colonies and by convection with the air and an equilibrium temperature will be reached.  
+
    <td bgcolor="#964141" width="800"><font color="#FFFFFF"><p>AOX pathway is responsible for thermogenesis in various organisms. But to what extent it would be responsible for heat production in genetically engineered bacteria remains an interesting question. Georgia Tech modeling team aimed at theorizing an answer to this question using both analytical and computational methods. The primary goal was to suggest a calorimetric technique with optimal sensitivity, as well as to compare heat transfer in liquid culture and bacterial
-
</p>
+
colonies.</p>
-
<p>
+
  </tr>
-
<center><img src="https://static.igem.org/mediawiki/2010/2/24/Modelingpic_2.png" alt="modeling picture"</center>
+
</table>
-
</p>
+
<table width="965" border="0" cellpadding="0" cellspacing="0">
-
<p>
+
  <tr>
-
Figure (B) presents the modeling of E. coli on a dry, plated culture in the form of a circuit diagram. The circuit diagram represents the CspA or HyBb promoter as a switch which would activate the expression of AOX pathway in the cell during cold shock. As the cell starts producing heat, mainly through AOX pathway, the heat starts to create a flux through the E. coli layer and release heat into the environment (basically in the media through conduction and in the air through convection). Since, the heat flows from E. coli in the media, it creates a temperature gradient across the whole plate due to the resistance encountered by it. These resistances are mainly due to conduction in media and convection in air which can be combined to single resistance as they are in parallel orientation to each other. 
+
    <td bgcolor="#7c1212" width="965"><font color="#FFFFFF"><p></p></font></td>
-
</p>
+
  </tr>
-
<p>
+
</table>
-
<a href="https://static.igem.org/mediawiki/2010/a/af/Modeling_heat_production_by_AOX.pdf"> Proposed model of heat generation via AOX in cells</a>
+
 
 +
<center><table width="900" border="0" cellpadding="2" cellspacing="10">
 +
  <tr>
 +
    <td bgcolor="#964141" width="800"><font color="#FFFFFF" size=4><p><center>Heat Transfer Modeling Aims</p>
 +
<ul>
 +
<li>Theoretic rate of heat production</li>
 +
<li>Calorimetric technique</li>
 +
<li>Heat transfer in liquid vs solid medium</li>
 +
<li>Analytical and computational methods</li>
 +
  </tr>
 +
</table>
 +
 
<hr>
<hr>
<img src="https://static.igem.org/mediawiki/2010/a/a2/Sponserslogo.jpg" alt="sponsors" width="970" border="0" usemap="#Map3" />
<img src="https://static.igem.org/mediawiki/2010/a/a2/Sponserslogo.jpg" alt="sponsors" width="970" border="0" usemap="#Map3" />

Revision as of 04:27, 27 October 2010

Retrieved from "http://2010.igem.org/Team:GeorgiaTech/Systems_Modeling"