"
Team:GeorgiaTech/Biobricks
From 2010.igem.org
BioBricks also known as standard biological parts, are DNA sequences that when introduced into an organism, act as a component of a desired mechanism. For more information on BioBricks visit iGEM's BioBrick page here. |
The construct above is a fusion of the HybB cold shock promoter, the Ompa signal peptide, and AOXa. |
The construct above contains with mRFP (red fluorescent protein) at the c-terminus of AOX will allow for imaging in the bacterial periplasm. |
To quantify the activity of the HyBb promoter at different temperatures, we will express mRFP using HyBb in construct above. Note that the final BioBricks will be in the pSB1C3 assembly plasmid. |
