Team:GeorgiaTech/Biobricks

From 2010.igem.org

(Difference between revisions)
 
(15 intermediate revisions not shown)
Line 4: Line 4:
<!--
<!--
 +
a, a:hover, a:visited{
 +
color: white;
 +
font-weight: bold;
 +
text-decoration: underline;
 +
}
 +
a:hover {
 +
color: black;
 +
}
body
body
{
{
Line 77: Line 85:
   <tr>
   <tr>
     <td bgcolor="#964141" width="800"><font color="#FFFFFF"><p>BioBricks also known as standard biological parts, are DNA sequences that when introduced into an organism, act as a component of a desired mechanism. For more information on BioBricks visit iGEM's BioBrick page<a href="http://partsregistry.org/Main_Page"> here</a>.
     <td bgcolor="#964141" width="800"><font color="#FFFFFF"><p>BioBricks also known as standard biological parts, are DNA sequences that when introduced into an organism, act as a component of a desired mechanism. For more information on BioBricks visit iGEM's BioBrick page<a href="http://partsregistry.org/Main_Page"> here</a>.
 +
  </tr>
 +
</table>
 +
<table width="965" border="0" cellpadding="0" cellspacing="0">
 +
  <tr>
 +
    <td bgcolor="#7c1212" width="965"><font color="#FFFFFF"><p></p></font></td>
   </tr>
   </tr>
</table>
</table>
Line 86: Line 99:
<p><center><font color="#FFFFFF" size=5><b>BioBrick Constructs</b></font></center></p>
<p><center><font color="#FFFFFF" size=5><b>BioBrick Constructs</b></font></center></p>
-
<center><img src="https://static.igem.org/mediawiki/2010/0/04/Biobrick_Constructs-1-.JPG" alt="construct" width="500" height="500"></center>
+
<center><img src="https://static.igem.org/mediawiki/2010/3/32/Ho.jpg" alt="construct" width="500" height="" img style="border: 2px solid black"></center>
 +
<center><table width="450" border="0" cellpadding="2" cellspacing="10">
 +
  <tr>
 +
    <td bgcolor="#964141" width="800"><font color="#FFFFFF"><p>The construct above is a fusion of the HybB cold shock promoter, the Ompa signal peptide, and AOXa. Aoxa is an alternative oxidase found in Sacred Lotus and shuffles electrons through an alternative path, thereby producing a thermogenesis event in Sacred Lotus. Our construct aims to produce an increase of heat above ambient temperature when cells expressing the construct are cold shocked (20c). The HybB promoter will express at lower temperatures (20 to 30c) but not at higher temperatures (37c).
 +
<p>More information can be found at the construct page: </p>
 +
<p>
 +
<a href="http://partsregistry.org/Part:BBa_K410000" title="Bba_K140000"> Part:BBa_K410000 </a>
 +
</p>
 +
  </tr>
 +
</table>
<table width="965" border="0" cellpadding="0" cellspacing="0">
<table width="965" border="0" cellpadding="0" cellspacing="0">
   <tr>
   <tr>
Line 92: Line 114:
   </tr>
   </tr>
</table>
</table>
-
<center><table width="900" border="0" cellpadding="2" cellspacing="10">
+
<table width="965" border="0" cellpadding="0" cellspacing="0">
   <tr>
   <tr>
-
     <td bgcolor="#964141" width="800"><font color="#FFFFFF"><p>There are several constructs we hope to submit as BioBricks to the Parts Registry. Construct (a) is a fusion of the HybB cold shock promoter, the Ompa signal peptide, and either of the two forms of AOX.  Construct (b) with mRFP (red fluorescent protein) at the c-terminus of AOX will allow for imaging in the bacterial periplasm. To quantify the activity of the HyBb promoter at different temperatures, we will express mRFP using HyBb in construct (c). Note that the final BioBricks will be in the pSB1C3 assembly plasmid. </p>
+
     <td bgcolor="#7c1212" width="965"><font color="#FFFFFF"><p></p></font></td>
   </tr>
   </tr>
</table>
</table>
 +
<center><img src="https://static.igem.org/mediawiki/2010/2/2f/Hoam.jpg" alt="construct" width="500" height="" img style="border: 2px solid black"></center>
 +
<center><table width="450" border="0" cellpadding="2" cellspacing="10">
 +
  <tr>
 +
    <td bgcolor="#964141" width="800"><font color="#FFFFFF"><p>The construct above containing mRFP (red fluorescent protein) at the c-terminus of AOX will allow for imaging in the bacterial periplasm.</p>
 +
  </tr>
 +
</table>
 +
<table width="965" border="0" cellpadding="0" cellspacing="0">
 +
  <tr>
 +
    <td bgcolor="#7c1212" width="965"><font color="#FFFFFF"><p></p></font></td>
 +
  </tr>
 +
</table>
 +
<table width="965" border="0" cellpadding="0" cellspacing="0">
 +
  <tr>
 +
    <td bgcolor="#7c1212" width="965"><font color="#FFFFFF"><p></p></font></td>
 +
  </tr>
 +
</table>
 +
<center><img src="https://static.igem.org/mediawiki/2010/3/35/Ho2.jpg" alt="construct" width="500" height="" img style="border: 2px solid black"></center>
 +
 +
<center><table width="450" border="0" cellpadding="2" cellspacing="10">
 +
  <tr>
 +
    <td bgcolor="#964141" width="800"><font color="#FFFFFF"><p>To quantify the activity of the HyBb promoter at different temperatures, we will express mRFP using HyBb in the construct above. Note that the final BioBricks will be in the pSB1C3 assembly plasmid.</p>
 +
  </tr>
 +
</table>
 +
<table width="965" border="0" cellpadding="0" cellspacing="0">
 +
  <tr>
 +
    <td bgcolor="#7c1212" width="965"><font color="#FFFFFF"><p></p></font></td>
 +
  </tr>
 +
</table>
 +
 +
<table width="965" border="0" cellpadding="0" cellspacing="0">
 +
  <tr>
 +
    <td bgcolor="#7c1212" width="965"><font color="#FFFFFF"><p></p></font></td>
 +
  </tr>
 +
</table>
 +
<center><img src="https://static.igem.org/mediawiki/2010/7/77/ControlIGEM_7_2010.png" alt="construct" width="500" height="" img style="border: 2px solid black" img style="border: 2px solid white"></center>
 +
 +
<center><table width="504" border="0" cellpadding="2" cellspacing="10">
 +
  <tr>
 +
    <td bgcolor="#964141" width="800"><font color="#FFFFFF"><p>CONTROL: Picture shows nonlocalized mRFP expression throughout E Coli.  They carry the biobrick sequence for mRFP expression (which is why they are red.) in psb1a3. They were grown overnight at 37C.</p>
 +
  </tr>
 +
</table>
 +
<table width="965" border="0" cellpadding="0" cellspacing="0">
 +
  <tr>
 +
    <td bgcolor="#7c1212" width="965"><font color="#FFFFFF"><p></p></font></td>
 +
  </tr>
 +
</table>
 +
 +
<table width="965" border="0" cellpadding="0" cellspacing="0">
 +
  <tr>
 +
    <td bgcolor="#7c1212" width="965"><font color="#FFFFFF"><p></p></font></td>
 +
  </tr>
 +
</table>
 +
<center><img src="https://static.igem.org/mediawiki/2010/5/51/ExpIGEM_8_2010.png" alt="construct" width="500" height="" img style="border: 2px solid black" img style="border: 2px solid white"></center>
 +
 +
<center><table width="504" border="0" cellpadding="2" cellspacing="10">
 +
  <tr>
 +
    <td bgcolor="#964141" width="800"><font color="#FFFFFF"><p>EXPERIMENTAL: Picture shows localized periplasmic mRFP expression in E coli.  These cells carry the coldshock promoter hybB, the targeting sequence for periplasmic expression ompA, and the red flourescent protein mRFP. This shows that we can direct the sequence to the periplasm. </p></center>
 +
  </tr>
 +
</table>
 +
<table width="965" border="0" cellpadding="0" cellspacing="0">
 +
  <tr>
 +
    <td bgcolor="#7c1212" width="965"><font color="#FFFFFF"><p></p></font></td>
 +
  </tr>
 +
</table>
 +
<hr>
<hr>
<img src="https://static.igem.org/mediawiki/2010/a/a2/Sponserslogo.jpg" alt="sponsors" width="970" border="0" usemap="#Map3" />
<img src="https://static.igem.org/mediawiki/2010/a/a2/Sponserslogo.jpg" alt="sponsors" width="970" border="0" usemap="#Map3" />

Latest revision as of 02:48, 28 October 2010