Team:GeorgiaTech/Biobricks

From 2010.igem.org

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<p><center><font color="#FFFFFF" size=5><b>BioBrick Constructs</b></font></center></p>
<p><center><font color="#FFFFFF" size=5><b>BioBrick Constructs</b></font></center></p>
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<center><img src="https://static.igem.org/mediawiki/2010/0/04/Biobrick_Constructs-1-.JPG" alt="construct" width="500" height="500"></center>
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<center><img src="https://static.igem.org/mediawiki/2010/3/32/Ho.jpg" alt="construct" width="500" height="" border="2"></center>
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     <td bgcolor="#964141" width="800"><font color="#FFFFFF"><p>There are several constructs we hope to submit as BioBricks to the Parts Registry. Construct (a) is a fusion of the HybB cold shock promoter, the Ompa signal peptide, and either of the two forms of AOX. Construct (b) with mRFP (red fluorescent protein) at the c-terminus of AOX will allow for imaging in the bacterial periplasm. To quantify the activity of the HyBb promoter at different temperatures, we will express mRFP using HyBb in construct (c). Note that the final BioBricks will be in the pSB1C3 assembly plasmid. </p>
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     <td bgcolor="#964141" width="800"><font color="#FFFFFF"><p>The construct above is a fusion of the HybB cold shock promoter, the Ompa signal peptide, and AOXa.</p>
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<center><img src="https://static.igem.org/mediawiki/2010/2/2f/Hoam.jpg" alt="construct" width="500" height="" border="2"></center>
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<center><table width="450" border="0" cellpadding="2" cellspacing="10">
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    <td bgcolor="#964141" width="800"><font color="#FFFFFF"><p>The construct above contains with mRFP (red fluorescent protein) at the c-terminus of AOX will allow for imaging in the bacterial periplasm.</p>
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<table width="965" border="0" cellpadding="0" cellspacing="0">
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    <td bgcolor="#7c1212" width="965"><font color="#FFFFFF"><p></p></font></td>
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<center><img src="https://static.igem.org/mediawiki/2010/3/35/Ho2.jpg" alt="construct" width="500" height="" border="2"></center>
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<table width="965" border="0" cellpadding="0" cellspacing="0">
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    <td bgcolor="#7c1212" width="965"><font color="#FFFFFF"><p></p></font></td>
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<center><table width="450" border="0" cellpadding="2" cellspacing="10">
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    <td bgcolor="#964141" width="800"><font color="#FFFFFF"><p>To quantify the activity of the HyBb promoter at different temperatures, we will express mRFP using HyBb in construct above. Note that the final BioBricks will be in the pSB1C3 assembly plasmid.</p>
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Revision as of 02:58, 26 October 2010