Team:Edinburgh/BioBricks

From 2010.igem.org

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   <th width="100px"><u>Link</u></th>
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   <td><b>cat</b></td>
   <td><b>cat</b></td>
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  <td align="left"><b>luxAB</b></td>
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  <td>Coding</td>
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  <td><a href="http://partsregistry.org/Part:BBa_K322139">BBa_K322139</a></td>
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  <td>Bacterial luciferase producing blue light.</td>
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   <td align="left"><b>Codon optimised bright firefly luciferase</b></td>
   <td align="left"><b>Codon optimised bright firefly luciferase</b></td>

Revision as of 15:10, 28 September 2010







BioBricks: Genomic BRIDGEs


For this part of the project we will have one main biobrick, which will be the construct required for the two-step markerless insertion, encompassing cat (chloramphenicol resistance) and sacB (prevents growth on sucrose).

Chloramphenicol resistance has already been well characterised in the registry, so our characterisation has mainly focused on sacB.

In addition we will probably be submitting the construct with the up- and down- stream sequences of useful genes which can be removed, e.g. tnaA, which produces indole - when removed E. coli no longer smells!


Name Type Link Description
cat Protein Domain BBa_K322210 chloramphenicol acetyltransferase (chloramphenicol resistance gene)
sacB Coding BBa_K322921 Bacillus subtilis levansucrase, lethal to E. coli in presence of sucrose




BioBricks: Bacterial BRIDGEs


***


Name Type Link Description
luxAB Coding BBa_K322139 Bacterial luciferase producing blue light.
Codon optimised bright firefly luciferase Coding BBa_K322451 Photinus pyralis luciferase mutated for increased bioluminescence.