Team:Edinburgh/Bacterial/Red light producer

From 2010.igem.org

(Difference between revisions)
Line 29: Line 29:
<ul class="dropdown dropdown-horizontal">
<ul class="dropdown dropdown-horizontal">
-
  <li style="width:13%;"><a href="https://2010.igem.org/Team:Edinburgh/Team" class="dir">team - illuminati</a>
+
  <li><a href="https://2010.igem.org/Team:Edinburgh" class="dir">home</a>
 +
</li>
 +
 
 +
<li><a href="https://2010.igem.org/Team:Edinburgh/Team" class="dir">team - illuminati</a>
   <ul>
   <ul>
   <li><a href="https://igem.org/Team.cgi?id=322">official</a></li>
   <li><a href="https://igem.org/Team.cgi?id=322">official</a></li>
Line 36: Line 39:
   <li><a href="https://2010.igem.org/Team:Edinburgh/Team/Students">students</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Team/Students">students</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Team/Environment">environment</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Team/Environment">environment</a></li>
 +
  <li><a href="https://2010.igem.org/Team:Edinburgh/Gallery" class="dir">gallery</a></li>
   </ul>
   </ul>
  </li>
  </li>
-
  <li style="width:14%;"><a href="https://2010.igem.org/Team:Edinburgh/Project" class="dir">genomic BRIDGEs</a>
+
  <li><a href="https://2010.igem.org/Team:Edinburgh/Project" class="dir">genomic BRIDGEs</a>
   <ul>
   <ul>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Project">the protocol</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Project">the protocol</a></li>
Line 49: Line 53:
  </li>
  </li>
-
  <li style="width:14%;"><a href="https://2010.igem.org/Team:Edinburgh/Bacterial" class="dir">bacterial BRIDGEs</a>
+
  <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial" class="dir">bacterial BRIDGEs</a>
   <ul>
   <ul>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Core_repressilator">the repressilator</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Bacterial/Core_repressilator">the repressilator</a></li>
Line 65: Line 69:
  </li>
  </li>
-
  <li style="width:15%;"><a href="https://2010.igem.org/Team:Edinburgh/Modelling" class="dir">modelling BRIDGEs</a>
+
  <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling" class="dir">modelling BRIDGEs</a>
   <ul>
   <ul>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling">the model</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Modelling">the model</a></li>
Line 74: Line 78:
  </li>
  </li>
-
  <li style="width:13%;"><a href="https://2010.igem.org/Team:Edinburgh/Human" class="dir">human BRIDGEs</a>
+
  <li><a href="https://2010.igem.org/Team:Edinburgh/Human" class="dir">human BRIDGEs</a>
   <ul>
   <ul>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Human">human aspects</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Human">human aspects</a></li>
Line 83: Line 87:
  </li>
  </li>
-
  <li style="width:10%;"><a href="https://2010.igem.org/Team:Edinburgh/Notebook" class="dir">notebooks&nbsp;&nbsp;&nbsp;&nbsp;</a>
+
  <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook" class="dir">lab notes&nbsp;&nbsp;&nbsp;</a>
   <ul>
   <ul>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook">collaboration</a></li>
   <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook">collaboration</a></li>
Line 96: Line 100:
  </li>
  </li>
   
   
-
  <li style="width:7%;"><a href="https://2010.igem.org/Team:Edinburgh/Gallery" class="dir">gallery&nbsp;&nbsp;&nbsp;&nbsp;</a>
+
  <li><a href="https://2010.igem.org/Team:Edinburgh/Acknowledgements" class="dir">acknowledgements</a>
-
</li>
+
-
 
+
-
<li style="width:14%;"><a href="https://2010.igem.org/Team:Edinburgh/Acknowledgements" class="dir">acknowledgements</a>
+
  </li>
  </li>

Revision as of 15:23, 10 August 2010








Firefly luciferase (EC 1.13.12.7) from Photinus pyralis is one of the most efficient bioluminescent proteins known. Its emission peak is about 557nm at pH 7.8 (this is the ordinary internal pH of E. coli during growth). We attempted to produce a mutant luciferase which would produce red light, in order to activate the red light sensor part (which responds optimally to 660nm light).

We used SDM on the wild type to produce different mutants:

  • Substitution mutant S248T
  • Insertion mutant 356K
  • Insertion mutant 356R