Team:Edinburgh/Bacterial/Red light producer

• The Illuminati
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• Genomic BRIDGEs
  • The Protocol
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• Bacterial BRIDGEs
  • The Repressilator
  • Red Sensor
  • Red Producer
  • Blue Sensor
  • Blue Producer
  • Green Sensor
  • Green Producer
  • The Project
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• Modelling BRIDGEs
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• Human BRIDGEs
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• Notebooks    
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  • BRIDGE
  • Red Light
  • Red Sensor
  • Blue Light
  • Blue Sensor
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• Acknowledgements

Firefly luciferase (EC 1.13.12.7) from Photinus pyralis is one of the most efficient bioluminescent proteins known. Its emission peak is about 557nm at pH 7.8 (this is the ordinary internal pH of E. coli during growth). We attempted to produce a mutant luciferase which would produce red light, in order to activate the red light sensor part (which responds optimally to 660nm light).

We used SDM on the wild type to produce different mutants:

• Substitution mutant S248T
• Insertion mutant 356K
• Insertion mutant 356R