From 2010.igem.org

Combined Model

Combined model. Coupled individual models for the simulation of the whole process and their interfaces. The concentration of CheYp determines the movement bias.

Interface

To combine light switch, chemotaxis and movement models, interfaces were defined. The combination was achieved in two steps:

• First, the deterministic light switch and chemotaxis models were combined by assuming a complete removal of a selected Che protein species by the light switch. The interface was defined as the concentration ([LSP-Che]) of this linked device.

• Second, the probabilistic movement model was combined with the light switch - chemotaxis model. To combine this models, the bias of CheYp was used to be the input parameter of the movement model; determining the probability of straight run / tumbling.

Combined model. Coupled individual models for the simulation of the whole process and their interfaces.

Challenges

The combination of the deterministic and probabilistic models was the biggest challenge in combining the models. The problem of different step-size could be solved by combination via Simulink and independent numerical integration of the two model types.