Team:ETHZ Basel/InformationProcessing/Visualization

From 2010.igem.org

(Difference between revisions)
(Visualization)
(Visualization)
Line 15: Line 15:
</html>
</html>
-
The cells were placed in a 50 &mu;m (?) high flow chamber ([[https://2010.igem.org/Team:ETHZ_Basel/InformationProcessing/MicroscopeSetup Details]]).  
+
The cells were placed in a 50 &mu;m (?) high flow chamber ([https://2010.igem.org/Team:ETHZ_Basel/InformationProcessing/MicroscopeSetup Details]).  
The movies were made out of bright field images. The excitation was 60ms, the period something around 1/7s, pretty much the fps rate of the movie (we can go heigher, but yet we already produced like 2 GB of raw images for every movie). Every image has the size of 1344 x 1024 pixels, 12 or 16 bit grayscale (I forgot what I have chosen, probably we will reduce it anyway later to speed up the imaging pipeline). For both movies the images were made out-of-focus to simplify cell detection.
The movies were made out of bright field images. The excitation was 60ms, the period something around 1/7s, pretty much the fps rate of the movie (we can go heigher, but yet we already produced like 2 GB of raw images for every movie). Every image has the size of 1344 x 1024 pixels, 12 or 16 bit grayscale (I forgot what I have chosen, probably we will reduce it anyway later to speed up the imaging pipeline). For both movies the images were made out-of-focus to simplify cell detection.

Revision as of 12:12, 16 September 2010

Visualization

The cells were placed in a 50 μm (?) high flow chamber (Details). The movies were made out of bright field images. The excitation was 60ms, the period something around 1/7s, pretty much the fps rate of the movie (we can go heigher, but yet we already produced like 2 GB of raw images for every movie). Every image has the size of 1344 x 1024 pixels, 12 or 16 bit grayscale (I forgot what I have chosen, probably we will reduce it anyway later to speed up the imaging pipeline). For both movies the images were made out-of-focus to simplify cell detection.