Team:ETHZ Basel/Biology

From 2010.igem.org

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As we plan to generate several fusion proteins with different linkers, we decided to use the cloning strategy BBF RFC 28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI (http://dspace.mit.edu/handle/1721.1/46721).
As we plan to generate several fusion proteins with different linkers, we decided to use the cloning strategy BBF RFC 28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI (http://dspace.mit.edu/handle/1721.1/46721).
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Currently we are working on putting all the BioBricks into a storage vector. The image [[Image:Constructs.jpg|200px|thumb|right|alt text]] shows all the constructs we plan to clone.
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<br>Currently we are working on putting all the BioBricks into a storage vector. The image [[Image:Constructs.jpg|200px|thumb|right|alt text]] shows all the constructs we plan to clone.

Revision as of 11:46, 28 July 2010

As we plan to generate several fusion proteins with different linkers, we decided to use the cloning strategy BBF RFC 28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI (http://dspace.mit.edu/handle/1721.1/46721).


Currently we are working on putting all the BioBricks into a storage vector. The image
alt text
shows all the constructs we plan to clone.