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Team:ETHZ Basel
From 2010.igem.org
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'''E-Lemming''' | '''E-Lemming''' | ||
| - | This years ETHZ Basel project goal is to control E.coli chemotaxis by hijacking and perturbing the tumbling / directed flagellar movement apparatus. By coupling directed flagellar movement regulating proteins to a light-sensitive spatial localization system, their activity can be controlled reversibly. A light-sensitive dimerizing complex fused to this regulating proteins and a spatial fixed location is induced by light pulses and therefore localization of the two molecules can be manipulated. | + | ETHZ Basel project goal is to control E. coli movements (chemotaxis) |
| + | by means of light. In fact, we will change the chemotaxis pathway | ||
| + | either by substituting the receptor with a light-sensitive one or by | ||
| + | interfering with the kinase-phosphatase process with proteins whose | ||
| + | binding and unbinding can be stimulated by pulses of light. In both | ||
| + | ways, E. coli tumbling is induced or removed just by pressing a light | ||
| + | switch and, as a consequence, a bacterium can be "driven" to a | ||
| + | precise, pre-fixed point. Tumbling / directed flagellar movement rates are supervised by image processing algorithms, which are linked to the light-pulse generator. This system enables to control single E.coli cells to move like mindless "Lemmings" in the direction they are forced to go. | ||
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| + | |||
| + | <!--- Old version | ||
| + | This years ETHZ Basel project goal is to control E.coli chemotaxis by hijacking and perturbing the tumbling / directed flagellar movement apparatus. By coupling directed flagellar movement regulating proteins to a light-sensitive spatial localization system, their activity can be controlled reversibly. A light-sensitive dimerizing complex fused to this regulating proteins and a spatial fixed location is induced by light pulses and therefore localization of the two molecules can be manipulated. ---> | ||
| + | |||
<!--- The Mission, Experiments ---> | <!--- The Mission, Experiments ---> | ||
Revision as of 09:12, 15 July 2010
Project abstract
E-Lemming
ETHZ Basel project goal is to control E. coli movements (chemotaxis) by means of light. In fact, we will change the chemotaxis pathway either by substituting the receptor with a light-sensitive one or by interfering with the kinase-phosphatase process with proteins whose binding and unbinding can be stimulated by pulses of light. In both ways, E. coli tumbling is induced or removed just by pressing a light switch and, as a consequence, a bacterium can be "driven" to a precise, pre-fixed point. Tumbling / directed flagellar movement rates are supervised by image processing algorithms, which are linked to the light-pulse generator. This system enables to control single E.coli cells to move like mindless "Lemmings" in the direction they are forced to go.
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