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| | {{ETHZ_Basel10}} | | {{ETHZ_Basel10}} |
| - | {{ETHZ_Basel10_Start}} | + | {{ETHZ_Basel10_Introduction}} |
| - | = WELCOME to the ETHZ Basel team wiki!= | + | = E. lemming - a remotely controlled living robot by ETH Zurich = |
| | + | {{ETHZ_Basel10_Teaser}} |
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| - | We are the team of ETH Zurich for iGEM 2010. Our goal is to remote control the movement of an individual Escherichia Coli bacterium.
| + | == Sponsors == |
| - | <br>In the classic video game [http://en.wikipedia.org/wiki/Lemmings_(video_game) '''Lemmings'''] the player has to lead a colony of lemmings with suicidal tendencies safely through a perilous world. Our project is about implementing the basic idea of this video game of spatial movement control into the biological system of Escherichia coli. | + | {| border="0" align="center" |
| - | | + | |<html><img src="https://static.igem.org/mediawiki/2010/6/65/ETHZ_Basel_sponsors.jpg"></html> |
| - | == E. lemming ==
| + | |} |
| - | [[Image:Setup.jpg|thumb|400px|'''Figure 1.''' Setup to control ''E. coli'' movements. An automatized microscope images the E. lemming. A connected computer system detects and tracks the cells. The direction of movement of the E. lemming is compared to the desired direction defined by the user, e.g. with a joystick. If the direction of movement deviates too much from the desired direction, the digital controller induces tumbling by sending a red light pulse. Otherwise, tumbling is repressed by sending a far-red light pulse.]]
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| - | The core idea of our project is to control chemotaxis of ''E. coli''-
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| - | by means of light! We'll realize this by hijacking and perturbing the tumbling / directed flagellar movement apparatus. By coupling directed flagellar movement regulating proteins to a '''novel synthetic light-sensitive spatial localization system''', their activity can be controlled reversibly. A light-sensitive dimerizing complex fused to this regulating proteins at a spatially fixed location is induced by light pulses and therefore localization of the two molecules can be manipulated. Tumbling / directed flagellar movement rates are monitored by image processing algorithms, which are linked to the light-pulse generator. This means that ''E. coli'' tumbling is induced or suppressed simply by pressing a light switch! This synthetic network enables control of single E. coli cells: '''We'll make them move like mindless "Lemmings"''' in the direction they are forced to go!
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