Team:Debrecen-Hungary/protocols
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=Welcome To Our Notebook= | =Welcome To Our Notebook= | ||
- | "Science is nothing else but the art of proper reproducibility. " <br> | + | <h1> "Science is nothing else but the art of proper reproducibility. " </h1> <br> |
The iGEM experience is not merely a project or a conference, but it was the way that most of our students got acquainted with the world of biological research laboratory. Pipettes, solutions, gels, electrodes, dishes and other scary machinery quickly filled our lives. | The iGEM experience is not merely a project or a conference, but it was the way that most of our students got acquainted with the world of biological research laboratory. Pipettes, solutions, gels, electrodes, dishes and other scary machinery quickly filled our lives. | ||
From day one we saw the vast importance of teaching our students to keep a proper laboratory journal. As time passed and our project grew., and with growth sprouted the idea of keeping an electronic laboratory journal with texts and video’s depicting the proper way of doing our niche of science. And so, we present to you our combined effort a text and video version | From day one we saw the vast importance of teaching our students to keep a proper laboratory journal. As time passed and our project grew., and with growth sprouted the idea of keeping an electronic laboratory journal with texts and video’s depicting the proper way of doing our niche of science. And so, we present to you our combined effort a text and video version | ||
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=== Transfection === | === Transfection === | ||
=== Ligand Treatment === | === Ligand Treatment === | ||
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+ | == Notebook protocols utilized by the Luciferase subteam == | ||
+ | |||
+ | === Measuring Luciferase activity with the Victor plate reader === | ||
</div> | </div> |
Revision as of 13:50, 28 September 2010
Welcome To Our Notebook"Science is nothing else but the art of proper reproducibility. "The iGEM experience is not merely a project or a conference, but it was the way that most of our students got acquainted with the world of biological research laboratory. Pipettes, solutions, gels, electrodes, dishes and other scary machinery quickly filled our lives. From day one we saw the vast importance of teaching our students to keep a proper laboratory journal. As time passed and our project grew., and with growth sprouted the idea of keeping an electronic laboratory journal with texts and video’s depicting the proper way of doing our niche of science. And so, we present to you our combined effort a text and video version ContentsNotebook protocols utilized by the bacterial work subteam Making Lurea's Broth - Transformation of competent cells - Mini Prep Notebook protocols utilized by the molecular tools subteam Restriction enzyme digestion - PCR Purification - Gel electrophoresis - Gel purification Notebook protocols utilized by the tissue culture subteam Cell Passaging - Media PEI Preparation - Transfection - Ligand Treatment Notebook protocols utilized by the Luciferase subteam Measuring Luciferase activity with the Victor plate reader Notebook protocols utilized by the bacterial work subteamMaking Lurea's BrothTransformation of competent cellsMini PrepNotebook protocols utilized by the molecular tools subteamRestriction enzyme digestionPCR product purificationGel electrophoresisGel purificationNotebook protocols utilized by the tissue culture subteamCell PassagingMedia PEI PreparationTransfectionLigand TreatmentNotebook protocols utilized by the Luciferase subteamMeasuring Luciferase activity with the Victor plate reader |