Team:Cambridge/Templates/boxesprototypenew

From 2010.igem.org

(Difference between revisions)

Latest revision as of 19:43, 7 June 2011

Abstract

We placed genes from fireflies and bioluminescent bacteria into E.coli. Codon optimisation and single amino acid mutagenesis allowed us to generate bright light output in a range of different colours. Future applications include and quantitative biosensors and biological alternatives to conventional lighting.

Multimedia

If you want a break from hard-core science, check out our Gibson Assembly music video.

You can also see view videos of our bacterial bubble lamp and project overview.

Tools

Gibson
Assembly Ligation
Calculator

@@ Line 5: / Line 5: @@
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 background: -moz-linear-gradient(top, #f2f2f2, #f8f8f8); color:#333333; height:150px;">
-<p style="line-height:120%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;">We placed genes from <a style="color:#6bbe00 !important" href="https://2010.igem.org/Team:Cambridge/Bioluminescence">fireflies</a> and bioluminescent <a style="color:#6bbe00 !important"  href="https://2010.igem.org/Team:Cambridge/Bioluminescence/Bacterial_Luciferases">prokaryotes</a> into E.coli to add brighter bacterial light to the registry.  We used site-directed mutagenesis to create a wide range of coloured bioluminescent BioBricks.</p>
+<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;"> We placed genes from <a style="color:#6bbe00 !important" href="https://2010.igem.org/Team:Cambridge/Bioluminescence">fireflies</a> and bioluminescent <a style="color:#6bbe00 !important"  href="https://2010.igem.org/Team:Cambridge/Bioluminescence/Bacterial_Luciferases">bacteria</a> into E.coli. Codon optimisation and single amino acid mutagenesis allowed us to generate bright light output in a range of different colours. Future applications include  and quantitative <a style="color:#6bbe00 !important" href="https://2010.igem.org/Team:Cambridge/Tools/microMeasure">biosensors</a> and biological <a style="color:#6bbe00 !important" href="https://2010.igem.org/Team:Cambridge/Tools/Lighting">alternatives</a> to conventional lighting.</p>
-<p style="line-height:120%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;">We  pioneered and documented the recently described <a style="color:#6bbe00 !important"  href="https://2010.igem.org/Team:Cambridge/Gibson/Introduction">Gibson Assembly</a> technique, building hardware and software tools to help future teams.</p></div>
+</div>
 </div>
@@ Line 14: / Line 14: @@
 <div id="ccontent" style="background:-webkit-gradient(linear, left top, left bottom, from(#f2f2f2), to(#f8f8f8));
 background: -moz-linear-gradient(top, #f2f2f2, #f8f8f8); color:#333333; height:150px;">
-<p style="line-height:120%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;">If you want a break from hard-core science, check out our Gibson Assembly  <a  style="color:#d9b400 !important" onclick="displayGibson('http://www.youtube.com/v/WCWjJFU1be8?fs=1&amp;hl=en_GB'); return false;" href="http://www.youtube.com/watch?v=WCWjJFU1be8">music video</a>.<br /><br />You can also see a video which shows off our <a style="color:#d9b400 !important" onclick="displayGibson('http://www.youtube.com/v/tUFscEVK5Ks?fs=1&amp;hl=en_GB'); return false;" href="http://www.youtube.com/watch?v=WCWjJFU1be8">bacterial bubble lamp</a>.
+<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;">If you want a break from hard-core science, check out our Gibson Assembly  <a  style="color:#d9b400 !important" onclick="displayGibson('http://www.youtube.com/v/WCWjJFU1be8?fs=1&amp;hl=en_GB'); return false;" href="http://www.youtube.com/watch?v=WCWjJFU1be8">music video</a>.<br /><br />You can also see view videos of our <a style="color:#d9b400 !important" onclick="displayGibson('http://www.youtube.com/v/tUFscEVK5Ks?fs=1&amp;hl=en_GB'); return false;" href="http://www.youtube.com/watch?v=WCWjJFU1be8">bacterial bubble lamp</a> and <a style="color:#d9b400 !important" onclick="displayGibson('http://www.cambridgeigem.org/video.php'); return false;" href="http://www.cambridgeigem.org/video.php?nojs=true">project overview</a>.
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@@ Line 30: / Line 39: @@
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-.twtr-hd{display:none;}
+Gibson<br />Assembly
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