Team:Cambridge/Templates/boxesprototypenew

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<div id="leftcontent" style="background:-webkit-gradient(linear, left top, left bottom, from(#f2f2f2), to(#f8f8f8));  
<div id="leftcontent" style="background:-webkit-gradient(linear, left top, left bottom, from(#f2f2f2), to(#f8f8f8));  
background: -moz-linear-gradient(top, #f2f2f2, #f8f8f8); color:#333333; height:150px;">
background: -moz-linear-gradient(top, #f2f2f2, #f8f8f8); color:#333333; height:150px;">
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<p style="line-height:120%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;">We placed genes from <a style="color:#6bbe00 !important" href="https://2010.igem.org/Team:Cambridge/Bioluminescence">fireflies</a> and bioluminescent <a style="color:#6bbe00 !important"  href="https://2010.igem.org/Team:Cambridge/Bioluminescence/Bacterial_Luciferases">prokaryotes</a> into E.coli to create the brightest bacterial light in the registryWe  pioneered and documented the recently described <a style="color:#6bbe00 !important" href="https://2010.igem.org/Team:Cambridge/Gibson/Introduction">Gibson Assembly</a> technique. We used site-directed mutagenesis to create a wide range of coloured bioluminescent BioBricks.</p></div>
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<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;"> We placed genes from <a style="color:#6bbe00 !important" href="https://2010.igem.org/Team:Cambridge/Bioluminescence">fireflies</a> and bioluminescent <a style="color:#6bbe00 !important"  href="https://2010.igem.org/Team:Cambridge/Bioluminescence/Bacterial_Luciferases">bacteria</a> into E.coli. Codon optimisation and single amino acid mutagenesis allowed us to generate bright light output in a range of different colours. Future applications include and quantitative <a style="color:#6bbe00 !important" href="https://2010.igem.org/Team:Cambridge/Tools/microMeasure">biosensors</a> and biological <a style="color:#6bbe00 !important" href="https://2010.igem.org/Team:Cambridge/Tools/Lighting">alternatives</a> to conventional lighting.</p>
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</div>
</div>
</div>
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<div id="ccontent" style="background:-webkit-gradient(linear, left top, left bottom, from(#f2f2f2), to(#f8f8f8));  
<div id="ccontent" style="background:-webkit-gradient(linear, left top, left bottom, from(#f2f2f2), to(#f8f8f8));  
background: -moz-linear-gradient(top, #f2f2f2, #f8f8f8); color:#333333; height:150px;">
background: -moz-linear-gradient(top, #f2f2f2, #f8f8f8); color:#333333; height:150px;">
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<p style="line-height:120%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;">If you want a break from hard-core science, check out our Gibson Assembly  <a  style="color:#d9b400 !important" onclick="displayGibson('http://www.youtube.com/v/WCWjJFU1be8?fs=1&amp;hl=en_GB'); return false;" href="http://www.youtube.com/watch?v=WCWjJFU1be8">music video</a>.<br /><br />You can also see a video which shows off our <a style="color:#d9b400 !important" onclick="displayGibson('http://www.youtube.com/v/tUFscEVK5Ks?fs=1&amp;hl=en_GB'); return false;" href="http://www.youtube.com/watch?v=WCWjJFU1be8">bacterial bubble lamp</a>.
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<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;">If you want a break from hard-core science, check out our Gibson Assembly  <a  style="color:#d9b400 !important" onclick="displayGibson('http://www.youtube.com/v/WCWjJFU1be8?fs=1&amp;hl=en_GB'); return false;" href="http://www.youtube.com/watch?v=WCWjJFU1be8">music video</a>.<br /><br />You can also see view videos of our <a style="color:#d9b400 !important" onclick="displayGibson('http://www.youtube.com/v/tUFscEVK5Ks?fs=1&amp;hl=en_GB'); return false;" href="http://www.youtube.com/watch?v=WCWjJFU1be8">bacterial bubble lamp</a> and <a style="color:#d9b400 !important" onclick="displayGibson('http://www.cambridgeigem.org/video.php'); return false;" href="http://www.cambridgeigem.org/video.php?nojs=true">project overview</a>.
<script>
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<div id="rightbox" style="float:left;  height:200px; margin-left:5px; width:242px;" class="tophome">
<div id="rightbox" style="float:left;  height:200px; margin-left:5px; width:242px;" class="tophome">
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<div id="rtop" style="background:#fb5c2b;background-image:url('https://static.igem.org/mediawiki/2010/a/ad/Cam-Gradient-Overlay.png')" class="secheader">Updates</div>
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<div id="rtop" style="background:#fb5c2b;background-image:url('https://static.igem.org/mediawiki/2010/a/ad/Cam-Gradient-Overlay.png')" class="secheader">Tools</div>
<div id="rcontent" style="background:-webkit-gradient(linear, left top, left bottom, from(#f2f2f2), to(#f8f8f8));  
<div id="rcontent" style="background:-webkit-gradient(linear, left top, left bottom, from(#f2f2f2), to(#f8f8f8));  
background: -moz-linear-gradient(top, #f2f2f2, #f8f8f8); color:#333333; height:150px; margin-top:5px; position:relative;">
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<script src="http://widgets.twimg.com/j/2/widget.js"></script>
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<div style="position:relative; top:10px;">
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<style>
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<a href="http://www.gibthon.org" id="leftbox" style="background:url('https://static.igem.org/mediawiki/2010/5/53/Cambridge-Gibthon.png'); position:absolute; width:120px; height:100px; left:0;top:0; background-repeat:no-repeat; display:block;text-align:center; padding-top:70px; background-position: center top; color:#e53500 !important;" >
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Gibson<br />Assembly
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Ligation<br />Calculator
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</a>
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<a href="http://twitter.com/camgem" style="position:absolute;z-index:100; left:10px; bottom:5px;"><img width="100" src="https://static.igem.org/mediawiki/2010/a/aa/Cambridge-twitter.png" /></a>
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<a href="http://facebook.com/cambridgeigem" style="position:absolute;z-index:100; right:10px; bottom:5px;"><img src="https://static.igem.org/mediawiki/2010/a/a1/Cambridge-facebook.png" style="border:2px solid #3b5998"/></a>
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</html>

Latest revision as of 19:43, 7 June 2011

Abstract

We placed genes from fireflies and bioluminescent bacteria into E.coli. Codon optimisation and single amino acid mutagenesis allowed us to generate bright light output in a range of different colours. Future applications include and quantitative biosensors and biological alternatives to conventional lighting.

Multimedia

If you want a break from hard-core science, check out our Gibson Assembly music video.

You can also see view videos of our bacterial bubble lamp and project overview.