Team:Cambridge/References/ProjectBioluminescence/Recovery

From 2010.igem.org

(Difference between revisions)
 
(8 intermediate revisions not shown)
Line 1: Line 1:
-
{{:Team:Cambridge/Templates/header}}
+
{{:Team:Cambridge/Templates/headerMinimalprototype}}
-
{{:Team:Cambridge/LumNavTemplate}}
+
{{:Team:Cambridge/Templates/RefBar}}
 +
{{:Team:Cambridge/Templates/headerbar|colour=#96d446|title=Bioluminescence: Luciferin Recovery}}
==LRE==
==LRE==
*[http://www.ncbi.nlm.nih.gov/pubmed/11457857 Paper on isolation of A-LRE]
*[http://www.ncbi.nlm.nih.gov/pubmed/11457857 Paper on isolation of A-LRE]
*[http://www.ncbi.nlm.nih.gov/nucleotide/14331151?report=genbank&log$=nucltop&blast_rank=1&RID=4EUAXMJW01R A-LRE (P.pyralis) Sequence]
*[http://www.ncbi.nlm.nih.gov/nucleotide/14331151?report=genbank&log$=nucltop&blast_rank=1&RID=4EUAXMJW01R A-LRE (P.pyralis) Sequence]
 +
* [http://www.ncbi.nlm.nih.gov/nuccore/AB072448.1 G-LRE (L. cruciata) Sequence]
*[http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TG1-506W6R3-1&_user=1495569&_coverDate=06%2F01%2F2010&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1409765644&_rerunOrigin=google&_acct=C000053194&_version=1&_urlVersion=0&_userid=1495569&md5=eec2ba99cb96a1e286d7ce14e8449b2a RACE-based amplification of cDNA and expression of a luciferin-regenerating enzyme (LRE): An attempt towards persistent bioluminescent signal]
*[http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TG1-506W6R3-1&_user=1495569&_coverDate=06%2F01%2F2010&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1409765644&_rerunOrigin=google&_acct=C000053194&_version=1&_urlVersion=0&_userid=1495569&md5=eec2ba99cb96a1e286d7ce14e8449b2a RACE-based amplification of cDNA and expression of a luciferin-regenerating enzyme (LRE): An attempt towards persistent bioluminescent signal]
Line 13: Line 15:
E. coli growth is impaired in the presence of micromolar amounts of D-cysteine. [http://www.ncbi.nlm.nih.gov/pubmed/11527960 Soutourina et al.] found that E. coli contain a D-cysteine desulfhydrase which can convert D-cysteine into pyruvate, H2S, and NH3. Overexpression of yedO (the E. coli gene encoding D-cysteine desulfydrase activity) protected E. coli against D-cysteine, whereas its inactivation rendered E. coli hypersensitive. With yedO intact, E. coli growth was improved by addition of D-cysteine as the sole sulfur source.  
E. coli growth is impaired in the presence of micromolar amounts of D-cysteine. [http://www.ncbi.nlm.nih.gov/pubmed/11527960 Soutourina et al.] found that E. coli contain a D-cysteine desulfhydrase which can convert D-cysteine into pyruvate, H2S, and NH3. Overexpression of yedO (the E. coli gene encoding D-cysteine desulfydrase activity) protected E. coli against D-cysteine, whereas its inactivation rendered E. coli hypersensitive. With yedO intact, E. coli growth was improved by addition of D-cysteine as the sole sulfur source.  
Similar to L-cysteine, D-cysteine exerts toxicity through inhibition of threonine deaminase, a key enzyme of the isoleucine, leucine, and valine biosynthesis pathway. Growth protection against D-cysteine in minimal medium was conferred by simultaneous addition of isoleucine, leucine and valine. L-aspartate was also observed to exert a protective effect against D-cysteine toxicity.
Similar to L-cysteine, D-cysteine exerts toxicity through inhibition of threonine deaminase, a key enzyme of the isoleucine, leucine, and valine biosynthesis pathway. Growth protection against D-cysteine in minimal medium was conferred by simultaneous addition of isoleucine, leucine and valine. L-aspartate was also observed to exert a protective effect against D-cysteine toxicity.
-
 
+
<html>
-
{{:Team:Cambridge/Templates/footer}}
+
</div>
 +
</html>
 +
{{:Team:Cambridge/Templates/footerMinimal}}

Latest revision as of 12:18, 7 October 2010