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Team:Cambridge/References/ProjectBioluminescence/Luciferase
From 2010.igem.org
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*[http://partsregistry.org/Part:BBa_G10001 parts registry lux operon] -currently waiting on an email to see how useful/available this part is | *[http://partsregistry.org/Part:BBa_G10001 parts registry lux operon] -currently waiting on an email to see how useful/available this part is | ||
*[[Team:Cambridge/ProjectBioluminescence/Luciferase/IMPORTANT INFO | IMPORTANT INFO]] | *[[Team:Cambridge/ProjectBioluminescence/Luciferase/IMPORTANT INFO | IMPORTANT INFO]] | ||
| + | * The Photorhabdus luminescens [http://partsregistry.org/Part:BBa_J70008 luxCDABE operon] in the registry. It's not well characterised | ||
[[Image:Luxoperondiagram.jpg]] | [[Image:Luxoperondiagram.jpg]] | ||
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==Plasmid experiment== | ==Plasmid experiment== | ||
Revision as of 14:36, 26 July 2010
Luciferase
Mutant luciferase (P. pyralis)
- Mutant Enhanced Photon Initiating Complex (EPIC) Luciferase (P.pyralis) Sequence
- Site-directed mutagenesis of firefly luciferase active site amino acids: a proposed model for bioluminescence color
- Improved practical usefulness of firefly luciferase by gene chimerization and random mutagenesis
Other Insect luciferases
- For colours we could use the click beetle luciferase red or green. They're reliable and from the Knight lab but not in the distribution.
Bacterial Bioluminescence
- Vibrio Fischeri
- Vibrio Harveyi
- Genetics of luxCDABE (adapted from wikipedia)
- 2006 attempts at BioBricking the lux operon
- Notes
- The lux wiki from parts registry
- NCBI Lux operon sequence
- parts registry lux operon -currently waiting on an email to see how useful/available this part is
- IMPORTANT INFO
- The Photorhabdus luminescens luxCDABE operon in the registry. It's not well characterised
Plasmid experiment
- Vibrio plasmid experiment -possible source of lux operon
- [1] - Transformation protocol for students from basics - includes several plasmids
- Plan
- set plasmid 555 into a BioBrick
- replace the promoter? sensitivity tuners?
Transformation Experiment to test Firefly luciferase in registry
- Firefly luciferase is in the registry as BBa_I712019, and have been sent to us as part of the spring 2010 distribution.
- We need to find a new vector with a bacterial ribosyme binding site, initiator, and a stop codon. Having searched via the catalog in the plasmids section, then in expression plasmids, we have selected a constitutive plasmid:
- The plasmid selected is BBa_J13002, which is a TetR repressed POPS/RIPS generator, again part of the spring 2010 distribution - kit plate 1, well 13B, plasmid pSB1A2. It was proven to work by UMN iGEM 2009.
