Team:Cambridge/References/ProjectBioluminescence/Luciferase

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(Mutant luciferase (P. pyralis))
(Transformation Experiment to test Firefly luciferase in registry)
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==Transformation Experiment to test Firefly luciferase in registry==
==Transformation Experiment to test Firefly luciferase in registry==
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* Firefly luciferase is in the registry as BBa_I712019, and have been sent to us as part of the spring 2010 distribution. We need to find a new vector with a bacterial ribosyme binding site, initiator, and a stop codon. Having searched via the catalog in the plasmids section, then in expression plasmids, we have selected a constitutive plasmid.
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* Firefly luciferase is in the registry as [http://partsregistry.org/Part:BBa_I712019 BBa_I712019], and have been sent to us as part of the spring 2010 distribution.  
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* The plasmid selected is BBa_JI3002, which is a TetR repressed POPS/RIPS generator, again part of the spring 2010 distribution - kit plate 1, well 13B, plasmid pSB1A2. It was proven to work by UMN iGEM 2009.
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*We need to find a new vector with a bacterial ribosyme binding site, initiator, and a stop codon. Having searched via the catalog in the plasmids section, then in expression plasmids, we have selected a constitutive plasmid.
 +
 
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* The plasmid selected is [http://www.partsregistry.org/Part:BBa_J13002 BBa_J13002], which is a TetR repressed POPS/RIPS generator, again part of the spring 2010 distribution - kit plate 1, well 13B, plasmid pSB1A2. It was proven to work by UMN iGEM 2009.
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Revision as of 11:03, 26 July 2010