"
Team:Cambridge/References/ProjectBioluminescence/LightLevel
From 2010.igem.org
Bioluminescence: Light Output
Increasing Light Emission
- Paper on isolation of G-LRE and H-LRE, which lists previous attempts to improve light production
- Intensified light emission in presence of low ATP concentration by PPDK
- Enhanced luciferase activity through cytodine nucleotides
- Gene chimerisation to improve practical usefulness of firefly luciferase
- Genetically modified firefly luciferase - the EPIC luciferase
- Bioluminescence Spectra of Native and Mutant Firefly Luciferases as a Function of pH
Relevant Physics
- The Candela measures how much light perceivable to the human eye is being emitted by a source of radiation, the Luminous Intensity (or luminous flux. It is related to Radiant intensity (or radiant flux) (which is measured in watts per steradian) but has been weighted by a Luminosity function. This alters the power output so that it is proportional to the sensitivity of the human eye: Definition of the candela with respect to Radiant intensity. Therefore, an object which is black may be emitting a lot of radiation (measured in watts per steradian) but since it is not perceivable to the human eye it is emitting 0cd of radiation.
- The lumen is simply cd*sr. It is thus the analogue to the Watt
- The Lux measures how much light is incident on a suface, the Illuminance. It is related to irradiance (measured in Watts per m^2) but has been weighted again by the luminosity function. 1lux=1cd/m^2
- The luminance of an object is how much light is emitted from it. This is also measured in Lux, but it is different contextually.
- A table of ambient illuminances. For our glowing bacteria to be seen from a given distance, they must be at least 1/20th of this value.
| Illuminance | Example |
|---|---|
| 10−5 lux | Light from Sirius, the brightest star in the night sky |
| 10−4 lux | Total starlight, overcast sky |
| 0.002 lux | Moonless clear night sky with airglow |
| 0.01 lux | Quarter moon |
| 0.27 lux | Full moon on a clear night |
| 1 lux | Full moon overhead at tropical latitude |
| 3.4 lux | Dark limit of civil twilight under a clear sky |
| 50 lux | Family living room |
| 80 lux | Hallway/toilet |
| 100 lux | Very dark overcast day |
| 320–500 lux | Office lighting |
| 400 lux | Sunrise or sunset on a clear day. |
| 1,000 lux | Overcast day |
| 10,000–25,000 lux | Full daylight (not direct sun) |
| 32,000–130,000 lux | Direct sunlight |
- A brief summary of the different luminous measures
- A site containing luminosity functions
- To summarise, the luminosity function allows one to convert between radiant energy and luminous (i.e., visible) energy.
Difficulties
Inclusion Bodies
Human Vision
Spectrum interpolation
- Radial-basis function network for the approximation of FBG sensor spectra with distorted peaks
- Splines
Source Code
