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Team:Cambridge/References/ProjectBioluminescence/Experiments
From 2010.igem.org
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==1. Test luminescence with the Luciferase from the registry== | ==1. Test luminescence with the Luciferase from the registry== | ||
| - | *Transform WT E.coli with the luciferase from the registry | + | * Transform WT E.coli with the luciferase from the registry. |
| - | + | * Feed with D-luciferin, develop a method for measuring light output | |
| - | + | * Variables - concentration of inducer (if luciferase promoter inducible), concentration of D-luciferin | |
| + | * Controls - no luciferin, no inducer | ||
| - | 2. Test use of CHBT as a substrate | + | ==2. Test use of CHBT as a substrate== |
| - | a) D-cysteine | + | *'''a) D-cysteine''' |
| + | * test | ||
b) L-cysteine via L-luciferin | b) L-cysteine via L-luciferin | ||
Revision as of 15:59, 26 July 2010
Firefly
1. Test luminescence with the Luciferase from the registry
- Transform WT E.coli with the luciferase from the registry.
- Feed with D-luciferin, develop a method for measuring light output
- Variables - concentration of inducer (if luciferase promoter inducible), concentration of D-luciferin
- Controls - no luciferin, no inducer
2. Test use of CHBT as a substrate
- a) D-cysteine
- test
b) L-cysteine via L-luciferin
