Team:Cambridge/References/ProjectBioluminescence
From 2010.igem.org
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*[http://www.ncbi.nlm.nih.gov/nucleotide/14331151?report=genbank&log$=nucltop&blast_rank=1&RID=4EUAXMJW01R A-LRE (P.pyralis) Sequence] | *[http://www.ncbi.nlm.nih.gov/nucleotide/14331151?report=genbank&log$=nucltop&blast_rank=1&RID=4EUAXMJW01R A-LRE (P.pyralis) Sequence] | ||
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*[http://www.ncbi.nlm.nih.gov/nuccore/AB261988.1 Mutant (EPIC) Luciferase (P.pyralis) Sequence] | *[http://www.ncbi.nlm.nih.gov/nuccore/AB261988.1 Mutant (EPIC) Luciferase (P.pyralis) Sequence] |
Revision as of 14:14, 22 July 2010
Bioluminescence
The Plan
- Characterise the transfer function of PoPs + CHBT --> Light
The Theory
- The Luciferin Cycle:
Increasing Light Emission
- Paper on isolation of G-LRE and H-LRE, which lists previous attempts to improve light production
- Intensified light emission in presence of low ATP concentration by PPDK
- Enhanced luciferase activity through cytodine nucleotides
- Gene chimerisation to improve practical usefulness of firefly luciferase
- Genetically modified firefly luciferase - the EPIC luciferase