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From 2010.igem.org

• The role of FIS in the Rcd checkpoint and stable maintenance of plasmid ColE1 ie good as the toggle control thingy
• Timing, self-control and a sense of direction are the secrets of multicopy plasmid stability
• The quiescent-cell expression system for protein synthesis in Escherichia coli
• ColE1 multimer formation triggers inhibition of Escherichia coli cell division.
• book about bac genomes
• D. Summers' Patent Application for Chem. Induction of Quiescence in Bacteria

Literally no documentation of h-ns and rcd in bacillus.

If we do quiescence, will have to be in e coli with bacillus as a side project. Have to get over IP issues with ucam + e coli. Implementation in ecoli will be fairly easy as it is a working system - all we need to do is brick it, and get it working.

Input from James

• Has to be done in E.Coli, will not work in non Coliform bacteria such as Bacilli.
• Switch on cell division would be very useful, however there are IP issues which must be discussed with David Summers.
• Another constraint is that liquid broth not agar must be used.
• Nobody has looked at it from a microfluidics point of view.
• When done before, the lambda phage takes 3 hours to initiate quiescence after the temperature change.
• The Lambda phage is the most stable to have an off switch, it does not express itself when needed (?)
• The RNA has a very long half life, it would be hard to strip away the system and put it under a new promotor, but, if possible would be extremely useful