Team:Cambridge/Quiescence

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(The aptamer-controlled-ribozyme approach)
(The aptamer-controlled-ribozyme approach)
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==The aptamer-controlled-ribozyme approach==
==The aptamer-controlled-ribozyme approach==
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[[Image:Rcdcomp hammerhead Rcd95.jpg|200px|left|thumb|mFold prediction for RNA construct structure not including aptamer]]
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[[Image:Rcdcomp hammerhead Rcd95.jpg|100px|left|thumb|mFold prediction for RNA construct structure not including aptamer]]
A new approach to making a quiescence switch would be to control the functionality of the folded RNA instead of its transcription, which proved to be problematic. Ideally the bacteria would grow in the lab or a vat in the presence of a ligand but would stop growing without dying, as soon as the ligand is removed. Thus if the engineered bacteria escaped into the wild they would stop growing very quickly as the ligand is diluted.  
A new approach to making a quiescence switch would be to control the functionality of the folded RNA instead of its transcription, which proved to be problematic. Ideally the bacteria would grow in the lab or a vat in the presence of a ligand but would stop growing without dying, as soon as the ligand is removed. Thus if the engineered bacteria escaped into the wild they would stop growing very quickly as the ligand is diluted.  

Revision as of 11:10, 28 July 2010