Team:Cambridge/OligoOrderVibrio13.08.10

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BioBrick Prefix:

If following part is coding or contains ATG:

gaattcgcggccgcttctag

Otherwise:

gaattcgcggccgcttctagag

BioBrick Suffix:

tactagtagcggccgctgcag

Pbad Forward Prefix Primer:

Sequence: gaattcgcggccgcttctagagTTATGACAACTTGACGG

Structure: one hairpin in gene

Tm: 52.95 deg

dG: 1.00 kcal/mol

Pbad Reverse Oend

Sequence: tagtcataccaacctcccttgcgGCTAGCCCAAAAAAAC

Structure: one hairpin in gene

Tm: 53.68 deg

dG: 1.11 kcal/mol

luxR forward primer Sequence: ATGAAAAACATAAATGCCG Tm: 58.06 deg dG: 1.59 kcal/mol

luxR reverse Iend Sequence: atgatcatcgccggcgacgtcCTCTAGTAGTGATCTACACTAGCACTATC Tm: 58.54 deg dG: 0.34 kcal/mol

luxI forward primer Sequence: AAGGGAGGTTGGTATGACTATA Tm: 58.52 deg dG: 2.41 kcal/mol

luxG reverse suffix primer (finishing before the final terminator) Sequence: atgatcatcgccggcgacgtcTTTATATTCTCTTTATACGTATGCAA Tm: 57.36 deg dG: -0.34 kcal/mol

luxO forward primer Sequence:AGGGAAAATAATGAATAAATGTATT Tm: 57.29 deg dG: 2.13 kcal/mol

luxO reverse suffix primer (finishing at RBS of luxG) Sequence: atgatcatcgccggcgacgtcAACTTAATCCTTGATATTCTTTTGTAT Tm: 57.96 deg dG: -0.34 kcal/mol

Pbad Reverse Rend

Sequence: ttcatctagtatttctcctctttGCTAGCCCAAAAAAAC

Structure: one hairpin in gene

Tm: 53.68 deg

dG: 1.83 kcal/mol

luxO forward prefix primer Sequence: gaattcgcggccgcttctagagAGGGAAAATAATGAATAAATGTAT Tm: 56.07 deg dG: 0.76 kcal/mol