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Team:Cambridge/Notebook/Week6
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Revision as of 23:06, 16 August 2010
Notebook: Week 6
Monday
In the morning we checked the BMG microplate reader which had been run over the weekend and provided interesting growth curves, quantifying the extent to which adding chloramphenicol to the medium slowed growth (this was by c. 50%). Bill miniprepped lux R DNA from the registry. James told us that to use the standard Gibson protocol we needed 40bp of overlap, not the 20bp we planned which required rethinking oligos. Peter prepared a number of cultures to test the effect of lumazine on emissions.
In the evening Hannah, Peter, Anja and Theo combined a take-away and beer with primer design, which went surprisingly well.
Tuesday
Wednesday
Thursday
Friday
Saturday
Sunday
