Team:Cambridge/Notebook/Week5

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We took photographs of the petri dishes that Theo and Peter had drawn.  These had grown up well, over a short period.  We believe this is because they were plated out with only ampicillin, and no chloramphenicol.
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[[Image:Cambridge-Glowth.jpg|650px]]
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Will designed oligos to be sent to Biolegio for synthesis.  Hannah and Emily wrote more messages on dishes, and planned a restriction digest to check for the biobrick compatibility of the V. fischeri lux operon.
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Anja and Theo miniprepped an overnight culture which had been inoculated with a ligation of an expression backbone and firefly luciferase.  They ran it on a gel, which showed that the insert had worked.  Discovered that E-gels can be used multiple times. Then they performed a very bodged luciferase assay.  0.5ml of cells were frozen and thawed three times to lyse them, then 0.05ml of a concentrated D-luciferin solution was added.  The camera showed them to be glowing.
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Latest revision as of 22:44, 18 August 2010