Team:Cambridge/Notebook/Week4

From 2010.igem.org

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==Monday==
==Monday==
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We transformed some of the competent TOP10 cells we made the previous week with plasmid phK724, which was kindly  sent to us by <a href="http://departments.kings.edu/biology/lux/">Jim Slock</a>
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We transformed some of the competent TOP10 cells we made the previous week with plasmid phK724, which was kindly  sent to us by [http://departments.kings.edu/biology/lux/ Jim Slock]
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We also placed E. coli containing phK555 in 5ml LB broth in a rotating 37°C incubator to grow up overnight.
We also placed E. coli containing phK555 in 5ml LB broth in a rotating 37°C incubator to grow up overnight.
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In terms of dry work, we discussed what we wanted to have synthesised.  We decided to dismiss PPDK (Pyruvate Orthophosphate Dikinase) and to include the Japanese firefly (<i>Luciola cruciata</i>) because of the ease with which we could change the colour of its emission.
In terms of dry work, we discussed what we wanted to have synthesised.  We decided to dismiss PPDK (Pyruvate Orthophosphate Dikinase) and to include the Japanese firefly (<i>Luciola cruciata</i>) because of the ease with which we could change the colour of its emission.

Revision as of 17:04, 14 August 2010