Team:Cambridge/Notebook/Week4

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==Sunday==
==Sunday==
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Went in to the lab on return:
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Found that:
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* TOP10 containing 5/7 had been successfully transformed, glowing
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* There were some colonies growing on ligation plate
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* Logos were mostly dim, except at the edges (weekend incubation too long)
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Theo and Peter:
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* Redrew logos from *very* turbid liquid culture of pHK555/724
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* Prepared overnight cultures of:
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** Two hns strains (details?)
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** The potential ligations
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** The firely luciferase, and backbones, for miniprep if necessary
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* + made up the 5mlsish of LB with pHK555/724 (Slock's strain) with 200ml LB
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Revision as of 23:34, 8 August 2010