Team:Cambridge/Gibson/Protocol

From 2010.igem.org

(Difference between revisions)
(Step 3: PCR)
(Step 3: PCR)
Line 57: Line 57:
|∞
|∞
|}
|}
 +
Line 67: Line 68:
<div align="center">
<div align="center">
<table class="vistable" padding="0" cellspacing="0">  
<table class="vistable" padding="0" cellspacing="0">  
 +
 +
<tr>
 +
<td>Step
 +
</td><td>Temp
 +
</td><td>Time
 +
</td></tr>
 +
<tr>
 +
<td>1:Initial Melting
 +
</td><td>98°C
 +
</td><td>30s
 +
</td></tr>
 +
<tr>
 +
<td>2:Melting
 +
</td><td>98°C
 +
</td><td>10s
 +
</td></tr>
 +
<tr>
 +
 +
<td>3:Annealing
 +
</td><td>T<sub>m</sub>°C
 +
</td><td>15s
 +
</td></tr>
 +
<tr>
 +
<td>4:Elongation
 +
</td><td>72
 +
</td><td>45s per kb DNA
 +
</td></tr>
 +
<tr>
 +
<td>5:GoTo step 2
 +
</td><td>
 +
</td><td>30 times
 +
</td></tr>
 +
<tr>
 +
 +
<td>6:Final Elongation
 +
</td><td>72°C
 +
</td><td>7m30
 +
</td></tr>
 +
<tr>
 +
<td>7:Final Hold
 +
</td><td>4°C
 +
</td><td>∞
 +
</td></tr>
   
   
-
<tr>
+
 
-
<td><b>Assembly Method</b>
+
 
-
</td><td><b>Number of steps to ligate N pieces of DNA</b>
+
 
-
</td></tr>
+
</table>  
-
<tr>
+
-
<td><a href="http://partsregistry.org/Assembly:Standard_assembly" class="external text" title="http://partsregistry.org/Assembly:Standard_assembly" rel="nofollow">Standard Assembly</a>
+
-
</td><td><a href="/Image:Steps-N.png" class="image" title="Image:Steps-N.png"><img alt="Image:Steps-N.png" src="/wiki/images/c/cc/Steps-N.png"  border="0" /></a>
+
-
</td></tr>
+
-
<tr>
+
-
<td><a href="http://partsregistry.org/Assembly:Rolling_assembly" class="external text" title="http://partsregistry.org/Assembly:Rolling_assembly" rel="nofollow">Parallel Assembly</a>
+
-
</td><td><a href="/Image:Steps-Log2.png" class="image" title="Image:Steps-Log2.png"><img alt="Image:Steps-Log2.png" src="/wiki/images/6/6f/Steps-Log2.png" width="138" height="21" border="0" /></a> (rounded up)
+
-
</td></tr>
+
-
<tr>
+
-
<td><a href="https://2010.igem.org/Team:Cambridge/Gibson/Mechanism" class="external text" title="https://2010.igem.org/Team:Cambridge/Gibson/Mechanism" rel="nofollow">Gibson Assembly</a>
+
-
</td><td><a href="/Image:Steps1.png" class="image" title="Image:Steps1.png"><img alt="Image:Steps1.png" src="/wiki/images/1/10/Steps1.png" width="83" height="18" border="0" /></a>
+
-
</td></tr></table>  
+
</div>
</div>
</html>
</html>

Revision as of 19:35, 24 October 2010