Preliminary description

The Cambridge iGEM team will be investigating bioluminescence and quiescence in E. coli. Our broad targets will be to:

• Maximise light output from bacterial or firefly luciferase
• Characterise a device with a known transfer function between PoPs input and light output
• Partially reconstruct the biosynthetic pathway of luciferin
• Create a biobrick for the induction of quiescence in E. coli
• Improve the existing switching mechanism for quiescence

Our focus will shift over the course of the project, and objectives may be added and removed, but the end goal ideally is to have a strain of E. coli which will stop growing and start emitting light at on a trigger.