Team:Cambridge/Bioluminescence/Colour

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We have created biobricks for Luciola cruciata luciferases of five different colours.   
We have created biobricks for Luciola cruciata luciferases of five different colours.   
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{| class="wikitable"
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<html>
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|-
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<table class="wikitable">
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| Part
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| Mutation
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<tr>
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| Colour
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<td width="100"> Part
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| λmax(nm)
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</td><td  width="100"> Mutation
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|-
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</td><td  width="100"> Colour
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| [http://partsregistry.org/Part:BBa_K325229 BBa_K325229
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</td><td  width="100"> λmax(nm)
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| Val239Ile
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</td></tr>
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| Green
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<tr>
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| 558
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<td> <a href="http://partsregistry.org/Part:BBa_K325229" class="external text" title="http://partsregistry.org/Part:BBa_K325229" rel="nofollow">BBa_K325229</a>
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|-
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</td><td> Val239Ile
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| [http://partsregistry.org/Part:BBa_K325209 BBa_K325209]
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</td><td> Green
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| WT
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</td><td> 558
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| Yellow/green  
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</td></tr>
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| 562
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<tr>
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|-
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<td> <a href="http://partsregistry.org/Part:BBa_K325209" class="external text" title="http://partsregistry.org/Part:BBa_K325209" rel="nofollow">BBa_K325209</a>
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| [http://partsregistry.org/Part:BBa_K325219 BBa_K325219]
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</td><td> WT
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| Ser286Asn
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</td><td> Yellow/green
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| Red/Orange
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</td><td> 562
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| 607
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</td></tr>
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|-
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<tr>
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| [http://partsregistry.org/Part:BBa_K325239 BBa_K325239]
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<td> <a href="http://partsregistry.org/Part:BBa_K325219" class="external text" title="http://partsregistry.org/Part:BBa_K325219" rel="nofollow">BBa_K325219</a>
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| Gly326Ser
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</td><td> Ser286Asn
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| Scarlet
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</td><td> Red/Orange
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| 609
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</td><td> 607
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|-
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</td></tr>
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| [http://partsregistry.org/Part:BBa_K325249 BBa_K325249]
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<tr>
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| His433Ser
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<td> <a href="http://partsregistry.org/Part:BBa_K325239" class="external text" title="http://partsregistry.org/Part:BBa_K325239" rel="nofollow">BBa_K325239</a>
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| Red
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</td><td> Gly326Ser
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| 612
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</td><td> Scarlet
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|-
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</td><td> 609
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| [http://partsregistry.org/Part:BBa_K325239 BBa_K325259]
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</td></tr>
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| Pro452Ser
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<tr>
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| Yellow
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<td> <a href="http://partsregistry.org/Part:BBa_K325249" class="external text" title="http://partsregistry.org/Part:BBa_K325249" rel="nofollow">BBa_K325249</a>
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| 595
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</td><td> His433Ser
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|}
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</td><td> Red
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</td><td> 612
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</td></tr>
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<tr>
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<td> <a href="http://partsregistry.org/Part:BBa_K325239" class="external text" title="http://partsregistry.org/Part:BBa_K325239" rel="nofollow">BBa_K325259</a>
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</td><td> Pro452Ser
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</td><td> Yellow
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</td><td> 595
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</td></tr></table>
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</html>
The bricks above are the luciferases with the Luciola cruciata luciferin regenerating enzyme under the control of an arabinose inducible promoter. The red/orange Ser286Asn mutant luciferase was also submitted with a promoter but no LRE and without a promoter or the LRE.  
The bricks above are the luciferases with the Luciola cruciata luciferin regenerating enzyme under the control of an arabinose inducible promoter. The red/orange Ser286Asn mutant luciferase was also submitted with a promoter but no LRE and without a promoter or the LRE.  
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{{:Team:Cambridge/Templates/footer}}

Revision as of 03:58, 28 October 2010