Team:Cambridge/Bioluminescence/Colour

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{{:Team:Cambridge/Templates/headerMinimalprototype}}{{:Team:Cambridge/Templates/headerbar|colour=#96d446|linkcolour=#6bbe00|title=Project Firefly: Coloured outputs}}
{{:Team:Cambridge/Templates/headerMinimalprototype}}{{:Team:Cambridge/Templates/headerbar|colour=#96d446|linkcolour=#6bbe00|title=Project Firefly: Coloured outputs}}
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{{:Team:Cambridge/Templates/RightImage|image=Cambridge-Mutagenesis.jpg|caption=Comparison of the colour of Luciola cruciata we ordered from DNA 2.0 and it's colour after a single amino acid substitution}}
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{{:Team:Cambridge/Templates/RightImage|image=Rainbow.png|caption=Comparison of the different coloured outputs produced over the course of the project}}
The peak emission wavelength of luciola cruciata luciferase can be altered by single amino acid changes as reported by [http://www.ncbi.nlm.nih.gov/pubmed/1946326 Kajiyama and Nakano]. Different coloured outputs, as well as being aesthetically pleasing, allow the most appropriate wavelength of output for a particular detector to be chosen. By putting different coloured luciferases under promoters which respond to different inputs, co-reporter assays would be possible.
The peak emission wavelength of luciola cruciata luciferase can be altered by single amino acid changes as reported by [http://www.ncbi.nlm.nih.gov/pubmed/1946326 Kajiyama and Nakano]. Different coloured outputs, as well as being aesthetically pleasing, allow the most appropriate wavelength of output for a particular detector to be chosen. By putting different coloured luciferases under promoters which respond to different inputs, co-reporter assays would be possible.

Revision as of 03:56, 28 October 2010