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Team:Cambridge/Bioluminescence/Bacterial Luciferases
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{{:Team:Cambridge/Templates/headerbar|colour=#fb5c2b|title=Bioluminescence: Bacterial Luciferases}} | {{:Team:Cambridge/Templates/headerbar|colour=#fb5c2b|title=Bioluminescence: Bacterial Luciferases}} | ||
{{:Team:Cambridge/Templates/RightImage|image=Cambridge-Photobacterium_plate.JPG|caption=One of the plates of <i>V. phosphoreum</i> we prepared.}} | {{:Team:Cambridge/Templates/RightImage|image=Cambridge-Photobacterium_plate.JPG|caption=One of the plates of <i>V. phosphoreum</i> we prepared.}} | ||
| + | '''Project Vibrio''' was designed to complement Project Firefly, by using bacterial lux operons to achieve some things that were not possible with firefly luciferase. | ||
| + | * Substrate production in E. coli, avoiding the need to add luciferin | ||
| + | * Emission of blue light | ||
| + | |||
==Pathway== | ==Pathway== | ||
Bacterial lux operons emit light using five enzymes: | Bacterial lux operons emit light using five enzymes: | ||
Revision as of 13:08, 21 October 2010
Bioluminescence: Bacterial Luciferases
One of the plates of V. phosphoreum we prepared.
Project Vibrio was designed to complement Project Firefly, by using bacterial lux operons to achieve some things that were not possible with firefly luciferase.
- Substrate production in E. coli, avoiding the need to add luciferin
- Emission of blue light
Pathway
Bacterial lux operons emit light using five enzymes:
- luxA and luxB form the luciferase part of the system, they emit light using the substrate tetradecanal
- luxC, luxD and luxE are involved in the biosynthesis of tetradecanal from readily available substrates
Bioluminescent species
The Cambridge team is looking into these bacterial luciferases:
- Vibrio fischeri forms symbioses with squid to prevent the squid from casting a shadow on moonlit nights. Due to this their lux proteins are non-functional above 30 degrees
- Vibrio (formerly Photobacterium) phosphoreum emits a very blue light, perhaps partly through its use of lumazine
