Team:Cambridge/BioBricks

From 2010.igem.org

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The Cambridge iGEM team has submitted 20 parts to the [http://partsregistry.org/Main_Page Registry of Standard Biological Parts].  Many of these parts have been extensively characterised. This data is stored in the Parts Registry, you can click on any BioBrick to view its technical datasheet.
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=Featured parts=
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<html>
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<style>
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td.lefttd{text-align:center; padding-right:20px;}
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table.partlist td{padding-bottom:40px;}
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</style>
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<table style="background:transparent" class="partlist">
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{{:Team:Cambridge/Templates/TableRowAll|3d=219|image=3/3c/Bulb219.png|title=Red luciferase and LRE operon from L. cruciata under pBAD|description=This part generates the luciferase and luciferin regenerating enzyme proteins from the Japanese firefly, <i>Luciola cruciata</i>, when induced by L-arabinose.  The luciferase produced has a point mutation Ser286Asn which gives it a redshifted emission spectrum as compared to wild-type.  The sequence of nucleotides is codon optimised for expression in E. coli.}}
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{{:Team:Cambridge/Templates/TableRowAll|3d=909|image=a/a6/G28.jpg|title=V. fischeri luxCDABE under pBAD|description=This part generates luxCDABE proteins from the bioluminescent bacterium, <i>Vibrio fischeri</i>, when induced by L-arabinose.  The BioBrick produces light from basic metabolites found in E. coli, luxCDE produce the substrate used by luxAB for bioluminescence.  The part creates a blue light.}}
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=Parts based on bacterial systems=
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<table style="background:transparent" class="partlist">
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{{:Team:Cambridge/Templates/Tablerow|3d=902|title=V. fischeri luxCD|description=This is a translational unit designed to produce <i>V. fischeri</i> luxC and luxD genes when placed under a suitable promoter. The DNA sequence is codon optimised for expression in E. coli. Both proteins are part of the  fatty aldehyde synthesis pathway.  luxC is a reductase and luxD is a acyltransferase.  LuxE must also be supplied to complete the pathway for fatty aldehyde synthesis.  Such fatty aldehydes are the substrate for the luxAB bacterial luciferase.}}
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{{:Team:Cambridge/Templates/Tablerow|3d=903|title=V. fischeri luxEG|description=This is a translational unit designed to produce <i>V. fischeri</i> luxE and luxG genes when placed under a suitable promoter. The DNA sequence is codon optimised for expression in E. coli. luxE functions as the synthetase creating fatty aldehydes for bioluminescence. LuxG is a flavin reductase.  }}
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{{:Team:Cambridge/Templates/Tablerow|3d=905|title=luxCDEG pLambda AB|description=This part constitutively expresses [http://partsregistry.org/wiki/index.php?title=Part:BBa_K216008 the Xenorhabdus luminescens luxAB luciferase] already in the registry.  It also features a translational unit for the substrate generating luxCDEG from Vibrio fischeri.  This means that only when supplied with PoPs will it produce light output. }}
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{{:Team:Cambridge/Templates/Tablerow|3d=906|title=pBAD luxCDEG pLambda AB|description=This is the same as the part above but was built for testing induction by adding the pBAD promoter, making light inducible by addition of L-arabinose. }}
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=Firefly luciferases=
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{{:Team:Cambridge/Templates/Tablerow|3d=101|title=Luciferase from Photinus pyralis with increased substrate affinity|description=This is a translational unit for a  mutated and codon-optimised form of the luciferase from the North American firefly, Photinus pyralis.  It is described in [http://www.ncbi.nlm.nih.gov/pubmed/17540326 Fujii et al. 2007] as having a 10 times higher substrate affinity and luminescence output compared to wildtype.}}
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{{:Team:Cambridge/Templates/Tablerow|3d=108|title=P.pyralis luciferase under pBAD|description=This is the same as the part above but placed under the pBAD promoter induced by L-arabinose for characterisation.}}
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{{:Team:Cambridge/Templates/Tablerow|3d=211|title=L. cruciata luciferase (red mutant)|description=This is a translational unit for a mutant (Ser286Asn) and codon-optimised luciferase from the Japanese firefly, Luciola cruciata. It is deeply red-shifted as compared to wild type. }}
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{{:Team:Cambridge/Templates/Tablerow|3d=218|title=L. cruciata luciferase under pBAD|description=This is the same as the part above but placed under the pBAD promoter induced by L-arabinose for characterisation.}}
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</table>
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=Firefly luciferin regenerating enzymes=
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<table style="background:transparent" class="partlist">
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{{:Team:Cambridge/Templates/Tablerow|3d=102|title=P. pyralis Luciferin Regenerating Enzyme|description=This is a translational unit for a  codon-optimised Luciferin Regenerating Enzyme (LRE) from the North American firefly, Photinus pyralis.}}
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{{:Team:Cambridge/Templates/Tablerow|3d=202|title=L. cruciata Luciferin Regenerating Enzyme|description=This is a translational unit for a  codon-optimised Luciferin Regenerating Enzyme (LRE) from the Japanese firefly, Luciola cruciata.}}
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</table>
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=Firefly luciferase, LRE operons=
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{{:Team:Cambridge/Templates/Tablerow|3d=210|title=Luciola cruciata LRE and luciferase|description=This translational unit combines the luciferin regenerating enzyme (BBa_K325202) and red-shifted luciferase (BBa_K325211) from the Japanese firefly, Luciola cruciata.  The luciferin regenerating enzyme helps to strengthen and sustain light output.}}
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{{:Team:Cambridge/Templates/Tablerow|3d=100|title=Photinus pyralis LRE and luciferase|description=This part combines the luciferin regenerating enzyme (BBa_K325102) and luciferase (BBa_K325101) from the North American firefly, Photinus pyralis.  The luciferin regenerating enzyme helps to strengthen and sustain light output.}}
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{{:Team:Cambridge/Templates/TableRowAll|3d=109|image=a/aa/Rainbow100.png|title=Photinus pyralis LRE and luciferase under pBAD|description=This part is the part above expressed under pBAD for characterisation.}}
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{{:Team:Cambridge/Templates/TableRowAll|3d=209|image=b/bc/Rainbow219.png|title=L. cruciata LRE and luciferase (wildtype) under pBAD|description=This part combines the Luciferin Regenerating Enzyme and luciferase from the Japanese firefly, Luciola cruciata.  It is expressed under pBAD.}}
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{{:Team:Cambridge/Templates/TableRowAll|3d=229|image=e/e9/Rainbow229.png|title=L. cruciata LRE and luciferase (green) under pBAD|description=This is the same as the part above but with a Val239Ile mutation which green-shifts its emission spectrum.}}
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{{:Team:Cambridge/Templates/TableRowAll|3d=239|image=4/4f/Rainbow239.png|title=L. cruciata LRE and luciferase (red) under pBAD|description=This is the same as the part above but instead has a Gly326Ser mutation which red-shifts its emission spectrum.}}
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{{:Team:Cambridge/Templates/TableRowAll|3d=249|image=d/d1/Rainbow249.png|title=L. cruciata LRE and luciferase (scarlet) under pBAD|description=This is the same as the part above but instead has a His433Tyr mutation which red-shifts its emission spectrum.}}
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{{:Team:Cambridge/Templates/TableRowAll|3d=259|image=4/47/Rainbow259.png|title=L. cruciata LRE and luciferase (yellow) under pBAD|description=This is the same as the part above but instead has a Pro452Ser mutation which shifts its emission spectrum to peak at a yellow wavelength.}}
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==Summary table==
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<groupparts>iGEM010 Cambridge</groupparts>
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Latest revision as of 03:09, 28 October 2010