Team:Cambridge/BioBricks

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{{:Team:Cambridge/Templates/Tablerow|3d=219|title=Red luciferase and LRE operon from L. cruciata under pBAD|description=This part generates the luciferase and luciferin regenerating enzyme proteins from the Japanese firefly, <i>Luciola cruciata</i>, when induced by L-arabinose.  The luciferase produced has a point mutation Ser286Asn which gives it a redshifted emission spectrum as compared to wild-type.  The sequence of nucleotides is codon optimised for expression in E. coli.}}
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{{:Team:Cambridge/Templates/TableRowAll|3d=219|image=3/3c/Bulb219.png|title=Red luciferase and LRE operon from L. cruciata under pBAD|description=This part generates the luciferase and luciferin regenerating enzyme proteins from the Japanese firefly, <i>Luciola cruciata</i>, when induced by L-arabinose.  The luciferase produced has a point mutation Ser286Asn which gives it a redshifted emission spectrum as compared to wild-type.  The sequence of nucleotides is codon optimised for expression in E. coli.}}
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{{:Team:Cambridge/Templates/Tablerow|3d=909|title=V. fischeri luxCDABE under pBAD|description=This part generates luxCDABE proteins from the bioluminescent bacterium, <i>Vibrio fischeri</i>, when induced by L-arabinose.  The BioBrick produces light from basic metabolites found in E. coli, luxCDE produce the substrate used by luxAB for bioluminescence.  The part creates a blue light.}}
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{{:Team:Cambridge/Templates/TableRowAll|3d=909|image=a/a6/G28.jpg|title=V. fischeri luxCDABE under pBAD|description=This part generates luxCDABE proteins from the bioluminescent bacterium, <i>Vibrio fischeri</i>, when induced by L-arabinose.  The BioBrick produces light from basic metabolites found in E. coli, luxCDE produce the substrate used by luxAB for bioluminescence.  The part creates a blue light.}}
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{{:Team:Cambridge/Templates/Tablerow|3d=210|title=Luciola cruciata LRE and luciferase|description=This translational unit combines the luciferin regenerating enzyme (BBa_K325202) and red-shifted luciferase (BBa_K325211) from the Japanese firefly, Luciola cruciata.  The luciferin regenerating enzyme helps to strengthen and sustain light output.}}
{{:Team:Cambridge/Templates/Tablerow|3d=210|title=Luciola cruciata LRE and luciferase|description=This translational unit combines the luciferin regenerating enzyme (BBa_K325202) and red-shifted luciferase (BBa_K325211) from the Japanese firefly, Luciola cruciata.  The luciferin regenerating enzyme helps to strengthen and sustain light output.}}
{{:Team:Cambridge/Templates/Tablerow|3d=100|title=Photinus pyralis LRE and luciferase|description=This part combines the luciferin regenerating enzyme (BBa_K325102) and luciferase (BBa_K325101) from the North American firefly, Photinus pyralis.  The luciferin regenerating enzyme helps to strengthen and sustain light output.}}
{{:Team:Cambridge/Templates/Tablerow|3d=100|title=Photinus pyralis LRE and luciferase|description=This part combines the luciferin regenerating enzyme (BBa_K325102) and luciferase (BBa_K325101) from the North American firefly, Photinus pyralis.  The luciferin regenerating enzyme helps to strengthen and sustain light output.}}
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{{:Team:Cambridge/Templates/Tablerow|3d=109|title=Photinus pyralis LRE and luciferase under pBAD|description=This part is the part above expressed under pBAD for characterisation.}}
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{{:Team:Cambridge/Templates/TableRowAll|3d=109|image=a/aa/Rainbow100.png|title=Photinus pyralis LRE and luciferase under pBAD|description=This part is the part above expressed under pBAD for characterisation.}}
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{{:Team:Cambridge/Templates/Tablerow|3d=209|title=L. cruciata LRE and luciferase (wildtype) under pBAD|description=This part combines the Luciferin Regenerating Enzyme and luciferase from the Japanese firefly, Luciola cruciata.  It is expressed under pBAD.}}
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{{:Team:Cambridge/Templates/TableRowAll|3d=209|image=b/bc/Rainbow219.png|title=L. cruciata LRE and luciferase (wildtype) under pBAD|description=This part combines the Luciferin Regenerating Enzyme and luciferase from the Japanese firefly, Luciola cruciata.  It is expressed under pBAD.}}
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{{:Team:Cambridge/Templates/Tablerow|3d=229|title=L. cruciata LRE and luciferase (green) under pBAD|description=This is the same as the part above but with a Val239Ile mutation which green-shifts its emission spectrum.}}
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{{:Team:Cambridge/Templates/TableRowAll|3d=229|image=e/e9/Rainbow229.png|title=L. cruciata LRE and luciferase (green) under pBAD|description=This is the same as the part above but with a Val239Ile mutation which green-shifts its emission spectrum.}}
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{{:Team:Cambridge/Templates/Tablerow|3d=239|title=L. cruciata LRE and luciferase (red) under pBAD|description=This is the same as the part above but instead has a Gly326Ser mutation which red-shifts its emission spectrum.}}
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{{:Team:Cambridge/Templates/TableRowAll|3d=239|image=4/4f/Rainbow239.png|title=L. cruciata LRE and luciferase (red) under pBAD|description=This is the same as the part above but instead has a Gly326Ser mutation which red-shifts its emission spectrum.}}
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{{:Team:Cambridge/Templates/Tablerow|3d=249|title=L. cruciata LRE and luciferase (scarlet) under pBAD|description=This is the same as the part above but instead has a His433Tyr mutation which red-shifts its emission spectrum.}}
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{{:Team:Cambridge/Templates/TableRowAll|3d=249|image=d/d1/Rainbow249.png|title=L. cruciata LRE and luciferase (scarlet) under pBAD|description=This is the same as the part above but instead has a His433Tyr mutation which red-shifts its emission spectrum.}}
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{{:Team:Cambridge/Templates/Tablerow|3d=259|title=L. cruciata LRE and luciferase (yellow) under pBAD|description=This is the same as the part above but instead has a Pro452Ser mutation which shifts its emission spectrum to peak at a yellow wavelength.}}
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{{:Team:Cambridge/Templates/TableRowAll|3d=259|image=4/47/Rainbow259.png|title=L. cruciata LRE and luciferase (yellow) under pBAD|description=This is the same as the part above but instead has a Pro452Ser mutation which shifts its emission spectrum to peak at a yellow wavelength.}}
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Latest revision as of 03:09, 28 October 2010