Team:Cambridge

From 2010.igem.org

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<div style="position:absolute; width:230px; left:5px; text-align:center;">A flask lit by E. coli transformed with one of our constructs </div>
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<div style="position:absolute; width:230px; left:240px; text-align:center;">E. coli transformed with our different coloured bioluminescent systems</div>
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<div style="position:absolute; width:220px; left:485px; text-align:center;">Team members illuminated only by our bacteria</div>
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Over the course of the summer we built a set of BioBricks to allow bioluminescence in a wide range of colours which have applications both as reporters for [https://2010.igem.org/Team:Cambridge/Tools/microMeasure '''biosensors'''] and as [https://2010.igem.org/Team:Cambridge/Tools/Lighting '''natural light sources''']. We also developed software tools to aid construction of BioBrick parts and devices.
{{:Team:Cambridge/Templates/Nolineheader2|header=Project Firefly}}
{{:Team:Cambridge/Templates/Nolineheader2|header=Project Firefly}}
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We are the  Cambridge Team for iGEM 2010. Over the course of the summer we have built a number of BioBricks to allow <strong>bioluminescence</strong>. We used genes originally found in fireflies (<em>Photinus pyralis</em> and <em>Luciola cruciata</em>) and those from the marine bacterium <em>Vibrio fischeri</em>.
 
We adopted a number of strategies to extend the use of '''firefly luciferase''':
We adopted a number of strategies to extend the use of '''firefly luciferase''':
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We complemented these firefly systems, which require the addition of the substrate luciferin, with light producing systems from '''Vibrio fischeri'''.  We believe we have created the [https://2010.igem.org/Team:Cambridge/Bioluminescence/G28 '''first BioBric'''k] to emit light in normal E. coli strains without the addition of any external substrate.
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We complemented these firefly systems, which require the addition of the substrate luciferin, with light producing systems from '''Vibrio fischeri'''.  We believe we have created the [https://2010.igem.org/Team:Cambridge/Bioluminescence/G28 '''first BioBrick'''] to emit light in normal E. coli strains without the addition of any external substrate.
{{:Team:Cambridge/Templates/Nolineheader2|header=Tools}}
{{:Team:Cambridge/Templates/Nolineheader2|header=Tools}}
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Over the summer we constructed a number of tools to assist the synthetic biologists of the future:
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During our project we made extensive use of [https://2010.igem.org/Team:Cambridge/Gibson/Introduction '''Gibson Assembly'''] to manufacture our parts, and have submitted an [https://2010.igem.org/Team:Cambridge/Gibson/RFC '''RFC'''] to the [http://bbf.openwetware.org/ '''BioBricks Foundation'''] to help future teams make best use of this technique.
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* '''[https://2010.igem.org/Team:Cambridge/Tools/Gibson Gibthon]''' allows the user to enter a series of BioBrick or GenBank IDs in a specific order and computes the appropriate primers for [https://2010.igem.org/Team:Cambridge/Gibson/Introduction '''Gibson Assembly'''].
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* [https://2010.igem.org/Team:Cambridge/Tools/GenBank BioBrick → GenBank] allows parts from the registry to be downloaded in .gb format, making them '''compatible''' with a wide range of biological software.
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* The [https://2010.igem.org/Team:Cambridge/Tools/Eglometer '''E.glometer'''] is a cheap, easily built, piece of electronics for measuring bioluminescence.  It allows scientists without access to expensive plate readers to measure bioluminescence.
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Along with this, we also constructed a number of tools to assist the synthetic biologists of the future:
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* [https://2010.igem.org/Team:Cambridge/Tools/Gibson '''Gibthon Construct Designer'''] allows the user to enter a series of BioBrick or GenBank IDs in a specific order and computes the appropriate primers for [https://2010.igem.org/Team:Cambridge/Gibson/Introduction '''Gibson Assembly'''].
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* [https://2010.igem.org/Team:Cambridge/Tools/GenBank '''BioBrick → GenBank'''] allows parts from the registry to be downloaded in .gb format, making them compatible with a wide range of biological software.
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* The [https://2010.igem.org/Team:Cambridge/Tools/Ligate '''Ligation Calculator'''] is a small calculator to help you work out the proportions to use for ligation in BioBrick assembly without having to worry about units.
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* The [https://2010.igem.org/Team:Cambridge/Tools/Eglometer '''E.glometer'''] is a cheap, easily built, piece of electronics for measuring bioluminescence.  It allows scientists without access to expensive plate readers to measure bacterial light output and has potential applications in [https://2010.igem.org/Team:Cambridge/Tools/microMeasure '''quantitative biosensors'''].
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{{:Team:Cambridge/Templates/Nolineheader2|header=Achievements in iGEM competition
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* Finalist
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* Winner of "Best Wiki" award (awarded jointly to Cambridge and [https://2010.igem.org/Team:Imperial_College_London '''Imperial College London'''])
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* Winner of the iGEMers Prize (awarded jointly to five iGEM teams)
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* Awarded a Gold Medal
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Please see [http://https://igem.org/Results '''iGEM official results page'''] to see how all the teams did.
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{{:Team:Cambridge/Templates/Nolineheader2|header=In the news
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* [http://www.newscientist.com/article/mg20827885.000-glowing-trees-could-light-up-city-streets.html New Scientist article]
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* [http://www.dailymail.co.uk/sciencetech/article-1333334/How-trees-glow-like-fireflies-day-replace-streetlights.html Daily Mail article]
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* [http://www.telegraph.co.uk/topics/christmas/8215302/The-science-of-Christmas-we-could-grow-our-own-fairy-lights-say-the-tree-wise-men.html The Telegraph Article]
[[Image:Cambridge_team_pictwo2010.jpg|center|frame|The team - in order - Anja Hohmann, Emily Knott, Hannah Copley, Will Handley, Theo Sanderson, Ben Reeve, Paul Masset, Peter Emmrich,  Bill Collins]]
[[Image:Cambridge_team_pictwo2010.jpg|center|frame|The team - in order - Anja Hohmann, Emily Knott, Hannah Copley, Will Handley, Theo Sanderson, Ben Reeve, Paul Masset, Peter Emmrich,  Bill Collins]]
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Latest revision as of 13:17, 26 December 2010