Team:Cambridge

From 2010.igem.org

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We are the  Cambridge Team for iGEM 2010. Over the course of the summer we have built a number of BioBricks to allow <strong>bioluminescence</strong>. We used genes originally found in fireflies (<em>Photinus pyralis</em> and <em>Luciola cruciata</em>) and those from the marine bacterium <em>Vibrio fischeri</em>.
We are the  Cambridge Team for iGEM 2010. Over the course of the summer we have built a number of BioBricks to allow <strong>bioluminescence</strong>. We used genes originally found in fireflies (<em>Photinus pyralis</em> and <em>Luciola cruciata</em>) and those from the marine bacterium <em>Vibrio fischeri</em>.
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We adopted a number of strategies to extend the use of '''firefly luciferase''':
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* [https://2010.igem.org/Team:Cambridge/Codons '''Codon optimisation'''] for increased light output
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* Use of a [https://2010.igem.org/Team:Cambridge/Bioluminescence/Luciferin_Regeneration '''luciferin regenerating enzyme'''].
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* Mutagenesis to create a number of [https://2010.igem.org/Team:Cambridge/Bioluminescence/Colour '''different colours''']
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We complemented these firefly systems, which require the addition of the substrate luciferin, with light producing systems from '''Vibrio fischeri'''.
These systems will form the basis of simple quantitative assays and in the future might also be used as a light source.
These systems will form the basis of simple quantitative assays and in the future might also be used as a light source.

Revision as of 15:50, 25 October 2010