Team:Caltech/Week 13

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==Monday 9/6==
==Monday 9/6==
 +
*Colony PCR cultures of L95 (HSP-LacI-EYFP) cells imaged under microscope
 +
**Cells are vastly overgrown and unhealthy (cultures visualized after one weekend)
 +
*Fresh cultures of L95 cells grown in preparation for a proper microscope imaging session
==Tuesday 9/7==
==Tuesday 9/7==
 +
*Digested successful sigma32 (HtpR) BioBrick in preparation for ligation into pSB1C3 chloramphenicol backbone
 +
**Treat the digestion like a backbone digestion
 +
*Ligated the digest into pSB1C3
 +
*Cleaned up the lab in preparation for mandatory leave of absence
==Wednesday 9/8==
==Wednesday 9/8==
 +
*Mandatory leave of absence begins
==Thursday 9/9==
==Thursday 9/9==

Revision as of 20:32, 20 September 2010


iGEM 2010



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Monday 9/6

  • Colony PCR cultures of L95 (HSP-LacI-EYFP) cells imaged under microscope
    • Cells are vastly overgrown and unhealthy (cultures visualized after one weekend)
  • Fresh cultures of L95 cells grown in preparation for a proper microscope imaging session

Tuesday 9/7

  • Digested successful sigma32 (HtpR) BioBrick in preparation for ligation into pSB1C3 chloramphenicol backbone
    • Treat the digestion like a backbone digestion
  • Ligated the digest into pSB1C3
  • Cleaned up the lab in preparation for mandatory leave of absence

Wednesday 9/8

  • Mandatory leave of absence begins

Thursday 9/9

Friday 9/10

 
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