Team:Caltech/Week 1

(Difference between revisions)

Latest revision as of 22:56, 16 July 2010

iGEM 2010

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GANTT Chart diagramming our planned progress throughout the summer.

Procured remaining antibiotics & sample of XL1 Blue cells
Prepare orders for Sigma, NEB, and others
- Sigma: Crosslinking compounds, substrates (linseed oil & soybean oil)
- NEB: Biobrick assembly kit, Ladders, polymerase
Prepared list of biobricks to order
- Addl Lysis bricks
- Agarase bricks

Made stock solutions for transformation and subsequent culturing
Transformed XL-1 Blue E. Coli with selected BioBricks
- J09250, K124017, K124014, R0082, B0015, B0034, I13601, pBAD 18 plasmid
Ordered more BioBricks:

(We also wanted to order sequencing primers G00100 & G00101, but apparently those aren't sent to teams.)

Checked transformation
Minipreped transformed bacteria
- Checked the plasmid concentration in the mini-prep solution
Made glycerol stocks from the transformed plasmid

Error creating thumbnail: Unable to create destination directory

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-[[Team:Caltech/Notebook|Back to Notebook]]
+{{:Team:Caltech/WeekTemplate}}
 ==Monday 6/14==
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 *Transformed XL-1 Blue E. Coli with selected BioBricks
 **[http://partsregistry.org/wiki/index.php?title=Part:BBa_J09250 J09250], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K124017 K124017], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K124014 K124014], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0082 R0082], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 B0015], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13601 I13601], pBAD 18 plasmid
+* Ordered more BioBricks:
+** [http://partsregistry.org/wiki/index.php?title=Part:BBa_K112012 K112012]
+** [http://partsregistry.org/wiki/index.php?title=Part:BBa_K108001 K108001]
+** [http://partsregistry.org/wiki/index.php?title=Part:BBa_K112806 K112806]
+** [http://partsregistry.org/wiki/index.php?title=Part:BBa_K284001 K284001]
+** [http://partsregistry.org/wiki/index.php?title=Part:BBa_I737020 I737020]
+(We also wanted to order sequencing primers [http://partsregistry.org/wiki/index.php?title=Part:BBa_G00100 G00100] & [http://partsregistry.org/wiki/index.php?title=Part:BBa_G00101 G00101], but apparently those aren't sent to teams.)
 ==Friday 6/18==
 *Checked transformation