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Team:Caltech/PlatingAntibiotics
From 2010.igem.org
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| - | == | + | ==Adding Antibiotics to LB-agar== |
| + | This method allows you to make LB-agar plates with no antibiotic, and add the antibiotic when needed. | ||
| + | |||
===Materials=== | ===Materials=== | ||
| - | * | + | * 1000x stock antibiotic solution |
| + | * LB-agar plates, clean | ||
| + | * Glass beads, sterile | ||
===Procedure=== | ===Procedure=== | ||
| - | # | + | # Warm plate to 37°C. |
| + | # Add 50μL of 1000x antibiotic to the surface of the agar. | ||
| + | # Distribute antibiotic evenly using glass beads. | ||
| + | # Dispose of beads and allow plates to dry in biohood or near flame. | ||
}} | }} | ||
Latest revision as of 23:14, 24 October 2010
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People
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 Adding Antibiotics to LB-agarThis method allows you to make LB-agar plates with no antibiotic, and add the antibiotic when needed. Materials
Procedure
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